SUR-8 Antibody - BSA Free

Western Blot: SUR-8 Antibody [NBP1-77041] - Jurkat cell lysate with at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry-Paraffin: SUR-8 Antibody [NBP1-77041] - Human spleen tissue at 5 ug/ml.

Western Blot: SUR-8 Antibody - BSA Free [NBP1-77041] - Western blot analysis of SUR-8 in Jurkat cell lysate with SUR-8 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SUR-8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SUR-8 Antibody - BSA Free (NBP1-77041) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human SHOC2. The immunogen is located within amino acids 30 - 80 of SHOC2. Amino Acid Squence: KESKEKEPKTKGKDAKDGKK
• Predicted Species: Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHOC2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  SUR-8 Peptide (NBP1-77041PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SUR-8 Antibody - BSA Free

• KIAA0862FLJ60412
• Protein soc-2 homolog
• Protein sur-8 homolog
• SIAA0862
• soc-2 (suppressor of clear, C.elegans) homolog
• soc-2 suppressor of clear homolog (C. elegans)
• SOC-2
• SOC2SUR8leucine-rich repeat protein SHOC-2
• SUR-8

Background

SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHOC2
• Uniprot:
  • NP_031399(Human)