SUMO1 Antibody (Y299)

Product Discontinued

SUMO1 Antibody (Y299) Summary

• Immunogen: A synthetic peptide corresponding to residues near the C-term of human SUMO-1 was used as immunogen.
• Localization: Nuclear, nuclear envelope and speckles. Also cytoplasmic.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUMO1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:250-500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:20
  • Western Blot 1:1000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 18/90kDa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A or G purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for SUMO1 Antibody (Y299)

• DAP1
• GAP modifying protein 1
• GAP-modifying protein 1
• GMP1SMT3CSMT3H3OFC10UBL1PIC1
• PIC1
• SENP2
• Sentrin
• small ubiquitin-related modifier 1
• SMT3 homolog 3
• SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 1 (yeast)
• SMT3
• SMT3C
• SMT3H3
• SUMO1
• SUMO-1
• Ubiquitin-homology domain protein PIC1
• ubiquitin-like 1 (sentrin)
• Ubiquitin-like protein SMT3C
• Ubiquitin-like protein UBL1
• UBL1

Background

Sumo 1 is a ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUMO 1 is also known to form a complex with RanGAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUMO1 Antibody (NB600-1100)(1)

Publication using NB600-1100

• Sun T, Gao F, Lin X et al. a-lipoic acid (a-LA) inhibits the transcriptional activity of interferon regulatory factor 1 (IRF-1) via SUMOylation Toxicol In Vitro. 2014-06-26 [PMID: 24975507] (IP, Human)
  
  Details:
  SUMO 1 antibody used for immunoprecipitation/IP on lysates of Human chondrocytes (treated or not with 50 uM alpha-lipoic acid) at a concentration of 5 ug antibody/1 mg whole cell lysates incubated ON 4C (Fig. 7)

Bioinformatics

• Gene Symbol: SUMO1
• Entrez:
  • 7341(Human)
  • 22218(Mouse)
  • 301442(Rat)
• Uniprot:
  • P63165()