![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220] - Left lane (-): 20 ug human HeLa whole cell protein. Right lane (+): 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with SUMO Activating Enzyme E1 (SAE1) Antibody (2 ug/mL) at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.](http://images.novusbio.com/fullsize/SUMO-Activating-Enzyme-E1-SAE1-UBA2-Antibody-Western-Blot-NBP2-44220-img0001.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NBP2-44220] - Left lane (-): 20 ug human HeLa whole cell protein. Right lane (+): 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with SUMO Activating Enzyme E1 (SAE1) Antibody (2 ug/mL) at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | This purified antibody is directed against human SUMO Activating Enzyme E1 (SAE1) protein. The product was purified from monospecific antiserum by affinity chromatography Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | This purified SUMO Activating Enzyme E1 (SAE1) Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human SUMO Activating Enzyme E1 (SAE1) (SAE1) protein. (Uniprot: Q9UBE0) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SAE1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~37 kDa in size corresponding to SAE1 protein by western blotting in the appropriate cell lysate or extract. |
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SUMO Activating Enzyme E1 (SAE1) Antibody (NBP2-44220)Find related products by research area.
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