SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

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Western Blot: SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody [NBP1-78069] - ) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody Summary

Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein.
Modification
p Ser185
Specificity
This purified antibody is directed against human SUMO Activating Enzyme E1 protein.?This antibody is specific for human SAE1 protein phosphorylated at S185.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAE1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:5000-1:25000
Application Notes
This purified antibody has been tested for use in ELISA and western blot.Expect a band at ~37 kDa in size corresponding - phosphorylated SAE1 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific antibody reacts with human SAE1 pS185 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Reactivity Notes

Reactive in human, bovine, mouse, rat, dog

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SUMO Activating Enzyme E1 (SAE1) [p Ser185] Antibody

  • AOS1
  • AOS1activator of SUMO1
  • FLJ3091
  • HSPC140
  • SAE1
  • SUA1
  • SUA1sentrin/SUMO-activating protein AOS1
  • SUMO Activating Enzyme E1 (SAE1/UBA2)
  • SUMO-1 activating enzyme E1 N subunit
  • SUMO1 activating enzyme subunit 1
  • SUMO-1 activating enzyme subunit 1
  • SUMO-activating enzyme subunit 1
  • UBA2
  • Ubiquitin-like 1-activating enzyme E1A
  • ubiquitin-like protein SUMO-1 activating enzyme
  • UBLE1A

Background

SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SAE1
Uniprot