SUCNR1/GPR91 Antibody

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of GPR91 (A135) antibody in extracts from HUVEC/MCF-7 cells.

Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GPR91 antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Immunohistochemistry-Paraffin: SUCNR1/GPR91 Antibody [NBP1-00861] - Staining of GPR91 in mouse Vas Deferens where strong membrane staining is observed.

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Analysis of human kidney tissue (A), mouse kidney tissue (B), rat kidney tissue (C), and hek293 cells (D) using GPR91 antibody at 2ug/ml.

Western Blot: SUCNR1/GPR91 Antibody [NBP1-00861] - Lane1:Hela whole cell lysate. Lane2:Mouse kidney tissue lysate. Lane3:Rat kidney tissue lysate.

Immunocytochemistry/Immunofluorescence: SUCNR1/GPR91 Antibody [NBP1-00861] - GRP1 antibody (1:50) was tested in HUVEC cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-P, In vitro
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

SUCNR1/GPR91 Antibody Summary

• Immunogen: A synthetic peptide made to an internal portion of the human GPR91 protein (between residues 100-200) [Uniprot: Q9BXA5]
• Specificity: GPR91 (A135) detects endogenous levels of GPR91 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SUCNR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • In vitro assay
• Application Notes: Use in In vitro reported in scientific literature (PMID: 30478422).
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adrenal Whole Tissue Lysate (Adult Whole Diabetes)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SUCNR1/GPR91 Antibody

• GPR91
• GPR91G protein-coupled receptor 91
• G-protein coupled receptor 91
• P2Y purinoceptor 1
• P2Y purinoceptor 1-like
• succinate receptor 1
• SUCNR1

Background

GPR91 (formerly known as P2U2) is a G protein-coupled, dicarboxylic acid succinate receptor with the highest level of expression in the kidney, predominantly in the proximal tubules and the lowest in the liver and the spleen. GPR91 functions as a citric acid cycle intermediate succinate receptor. Two signaling pathways result from GPR91 activation, the pertussis-toxin-sensitive Gi/Go pathway and the pertussis-toxin-insensitive Gq pathway. Four amino acid residues are necessary for GPR91 activation by succinate: Arg 99, His 103, Arg 252 and Arg 281. GPR91 plays an important role in the succinate-induced hypertensive effect and may be involved in renovascular hypertension, a disease linked to diabetes, renal failure and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUCNR1/GPR91 Antibody (NBP1-00861)(13)

Publications using NBP1-00861

• Wu JY, Huang TW, Hsieh YT et al. Cancer-Derived Succinate Promotes Macrophage Polarization and Cancer Metastasis via Succinate Receptor Mol. Cell Nov 5 2019 [PMID: 31735641]
• Peruzzotti-Jametti L, Bernstock JD, Vicario N et al. Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation. Cell Stem Cell. Mar 1 2018 [PMID: 29478844] (WB)
• Nguyen G, Park SY, Le CT et al. Metformin ameliorates activation of hepatic stellate cells and hepatic fibrosis by succinate and GPR91 inhibition. Biochem. Biophys. Res. Commun. 2017 Dec 23 [PMID: 29278707] (Human)
• Yang L, Yu D, Fan Hh et al. Triggering the succinate receptor GPR91 enhances pressure overload-induced right ventricular hypertrophy. Int J Clin Exp Pathol. 2014 Oct 22 [PMID: 25337184] (ICC/IF, Rat)
• Li T, Hu J, Du S et al. ERK1/2/COX-2/PGE2signaling pathway mediates GPR91-dependent VEGF release in streptozotocin-induced diabetes. Molecular Vision. 2014 Jul 31 [PMID: 25324681] (WB, ICC/IF, Rat)
• Li T, Hu J, Du S et al. ERK1/2/COX-2/PGE2 signaling pathway mediates GPR91-dependent VEGF release in streptozotocin-induced diabetes. Molecular Vision. 2014 Jul 31 (ICC/IF)
• Wang T, Xu YQ, Yuan YX et al. Succinate induces skeletal muscle fiber remodeling via SUNCR1 signaling pathway EMBO Rep. Jul 18 2019 [PMID: 31318145] (WB, Mouse)
• Caielli S, Veiga DT, Balasubramanian P et al. A CD4+ T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate. Nat. Med. Nov 26 2018 [PMID: 30478422] (In vitro, Human)
• Guo Y, Xie C, Li X et al. Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis. Nat Commun. 2017 May 31 [PMID: 28561074] (ICC/IF, Mouse)
• Li Tingting, Hu Jianyan, Gao Fengjuan et al. Transcription factors regulate GPR91-mediated expression of VEGF in hypoxia-induced retinopathy. Scientific Reports 2017 [PMID: 28374767] (ICC/IF, Human)
• Mu X, Zhao T, Xu C et al. Oncometabolite succinate promotes angiogenesis by upregulating VEGF expression through GPR91-mediated STAT3 and ERK activation. Oncotarget. Feb 21 2017 [PMID: 28061458] (WB, Human)
• Hu J, Li T, Du S et al. The MAPK signaling pathway mediates the GPR91-dependent release of VEGF from RGC-5 cells. Int. J. Mol. Med. 2015 Apr 23 [PMID: 25936351] (ICC/IF, Rat)
  
  Details:
  SUCNR1/GPR91 antibody was used for ICC-IF analysis of rat's retinal ganglion cell line RGC-5 cells. The assay implicated permeablization of cells with 0.1% Triton X-100 for 20 min at 4C, blocking with 5% BSA for 1 h at RT, use of primary antibody at 1:200 dilution with ON 4C incubation.
• Hu J, Wu Q, Li T, Chen Y, Wang S. Inhibition of high glucose-induced VEGF release in retinal ganglion cells by RNA interference targeting G protein-coupled receptor 91. Exp Eye Res 2013 Feb 1 [PMID: 23379999] (WB, ICC/IF, Rat)

FAQs for SUCNR1/GPR91 Antibody (NBP1-00861). (Showing 1 - 1 of 1 FAQs).

1. I am looking for a reliable GPR91 antibody suitable for immunohistochemistry and FACS. I know the Saphieia group from Montreal who used your GPR91 antibody in the past. Sadly they told me that that particular antibody is no longer available. Please can you advise me whether the new antibody NBP1-00861 truly does work for IHC/IF.
   • Yes, a couple of our other GPR91 antibodies were discontinued last year and at the moment we have only one GPR91 antibody (#NBP1-00861) which is one of our best selling products without even a single complaint. In our lab, we have tested GPR91-Antibody NBP1-00861 for WB and ICC/IF applications in Human, Mouse & Rat species. We have not tested this antibody in IHC and FLOW applications yet. However, some of our customers have used this antibody for IHC application and you may see following publications for that: Vargas et al. J Am Soc Nephrol. 2009 May;20(5):1002-11; Robben et al. Kidney Int. 2009 Dec;76(12):1258-67. Moreover, Hu et al (Exp Eye Res. 2013 Apr;109:31-9) and Aguiar et al. (Cell Calcium. 2010 Jan;47(1):37-46) have cited the ICC-IF use of this product. Because this antibody worked well in ICC-IF application and it is compatible with IF based immunodetection methods, I strongly believe that this antibody should work in FLOW application also. If you would be interested in testing this novel application, please take a look at our Innovator's Reward program.

Bioinformatics

• Gene Symbol: SUCNR1
• Entrez:
  • 56670(Human)
  • 408199(Rat)
• Uniprot:
  • Q9BXA5(Human)