Substance P Parameter Assay Kit

• Reactivity: All-Multi
• Applications: ELISA
• Conjugate: Biotin

Substance P Parameter Assay Kit Summary

• Background: The Parameter Substance P Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure Substance P in cell culture supernates, serum, plasma, saliva, and urine. It contains a synthetically derived human Substance P peptide and has been shown to accurately quantitate this peptide. Results obtained using samples containing natural Substance P showed linear curves that were para...llel to the standard curves obtained using the Parameter kit standards. These results indicate that this kit can be used to determine relative mass values for natural Substance P.
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• Specificity: Measures Substance P levels in various samples
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Substance P Parameter Assay Kit

• neurokinin 1
• neurokinin 2
• neurokinin A
• Neurokinin-1
• neuromedin L, neurokinin alpha, neuropeptide K, neuropeptide gamma)
• neuropeptide gamma
• neuropeptide K
• NK2
• NKA
• NKNAneurokinin alpha
• NPK
• PPT
• protachykinin-1
• substance K
• Substance P
• TAC1
• TAC2neuromedin L
• tachykinin 2
• tachykinin, precursor 1 (substance K, substance P, neurokinin 1, neurokinin 2
• tachykinin, precursor 1

Background

Substance P (SP, Neurokinin-1), is member of the Tachykinin peptide family and binds primarily to the Neurokinin 1 Receptor (NK1R, Substance P receptor, Tachykinin receptor 1) and with low affinity to the NK2 and NK3 receptors. Substance P is processed from preproTachykinin a, b, g and d isoforms that also generate other Tachykinins Neuropeptide K, Neurokinin A, Neurokinin B, and Neuropeptide G. Substance P is produced in the central nervous system, primary sensory neurons (C-type fibers), and endocrine cells of the intestinal mucosa. It triggers pain signals in the spinal cord dorsal horn as well as hypotension, tachycardia, release of inflammatory mediators, and direct antimicrobial action.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Substance P (KGE007)(40)

Publications using KGE007

• Li, W;Wang, S;Zhang, T;Zhu, Y;Yu, L;Xu, X; Activation of Adenosine Triphosphate-Gated Purinergic 2 Receptor Channels by Transient Receptor Potential Vanilloid Subtype 4 in Cough Hypersensitivity Biomolecules 2025-02-14 [PMID: 40001589] (Guinea Pig)
• Yoon, HJ;Moon, JY;Kim, HJ;Park, S;Choi, JS;Choi, HI;Kim, S;Yoon, KC; Evaluation of Ocular Irritation Sensitivity: Implications of Clinical Parameters, Pain Sensitivity, and Tear Neuromediator Profiles Journal of clinical medicine 2024-12-29 [PMID: 39797220] (Human)
• Kinugasa, F;Kajikawa, S;Weng, J;Ugawa, T;Fushiki, H;Yamanaka, Y;Nagata, M;Haggerty, G;Akuzawa, S;Nakazawa, T;Suzuki, H;Sawamoto, T; Effect of antiemetics on zolbetuximab-induced gastric injury and emesis in ferrets Journal of pharmacological sciences 2024-11-01 [PMID: 39313274] (Ferret)
• Georgiou, SG;Anagnostou, TL;Sideri, AI;Gouletsou, PG;Athanasiou, LV;Kazakos, G;Tsioli, V;Dermisiadou, E;Galatos, AD; Effect of classical music on light-plane anaesthesia and analgesia in dogs subjected to surgical nociceptive stimuli Scientific reports 2024-08-22 [PMID: 39174615] (Canine)
• Niebrzydowska-Tatus, M;Pe?ech, A;Bie?, K;Rekowska, AK;Doma?ska, A;Kimber-Trojnar, ?;Leszczy?ska-Gorzelak, B;Trojnar, M; Substance P Concentration in Gestational Diabetes and Excessive Gestational Weight Gain and Its Impact on Neonatal Anthropometry International journal of molecular sciences 2024-03-28 [PMID: 38612572] (Human)
• de Souza, S;Rosario Claudio, J;Sim, J;Inyang, KE;Dagenais, A;Monahan, K;Lee, B;Ramakrishnan, H;Parmar, V;Geron, M;Scherrer, G;Folger, JK;Laumet, G; Interleukin-10 signaling in somatosensory neurons controls CCL2 release and inflammatory response Brain, behavior, and immunity 2023-12-09 [PMID: 38081433] (Mouse)
• Q Chen, R Dong, L Zhang, M Zhang, X Xu, L Yu, Z Qiu Tracheobronchial-esophageal reflex initiates esophageal hypersensitivity and aggravates cough hyperreactivity in guinea pigs with esophageal acid infusion Respiratory physiology & neurobiology, 2022-03-28;301(0):103890. 2022-03-28 [PMID: 35358761] (Guinea Pig)
• JH Park, CH Lee, HD Ham, ES Choi, C Lee, S Lee Analgesic effects of a 5-HT3 receptor antagonist in an animal model of complex regional pain syndrome European review for medical and pharmacological sciences, 2021-11-01;25(22):7051-7057. 2021-11-01 [PMID: 34859869] (Rats)
• J Cheng, Z Xie, S Wang, S Wen, S Niu, C Shi, L Yu, X Xu Cough hypersensitivity in patients with metabolic syndrome: a clinical finding and its possible mechanisms BMC pulmonary medicine, 2021-09-06;21(1):284. 2021-09-06 [PMID: 34488706] (Human)
• F Corrigan, I Cernak, K McAteer, SC Hellewell, JV Rosenfeld, RJ Turner, R Vink NK1 antagonists attenuate tau phosphorylation after blast and repeated concussive injury Scientific Reports, 2021-04-23;11(1):8861. 2021-04-23 [PMID: 33893374] (Rat)
• YC Liu, GH Yam, MT Lin, E Teo, SK Koh, L Deng, L Zhou, L Tong, JS Mehta Comparison of tear proteomic and neuromediator profiles changes between small incision lenticule extraction (SMILE) and femtosecond laser-assisted in-situ keratomileusis (LASIK) Journal of advanced research, 2020-11-05;29(0):67-81. 2020-11-05 [PMID: 33842006] (Human)
• SE Lee, SJ Lee, SE Kim, K Kim, B Cho, K Roh, SC Kim Intravenous allogeneic umbilical cord blood-derived mesenchymal stem cell therapy in recessive dystrophic epidermolysis bullosa patients JCI Insight, 2021-01-25;6(2):. 2021-01-25 [PMID: 33491668] (Human)
• X Zeng, C Xu, X Xu, Y Zhang, Y Huang, X Huo Elevated lead levels in relation to low serum neuropeptide Y and adverse behavioral effects in preschool children with e-waste exposure Chemosphere, 2020-12-21;269(0):129380. 2020-12-21 [PMID: 33383249] (Human)
• A Yamada, Y Sato, T Horike, M Miyanaga, DH Wang Effects of a Forest Walk on Urinary Dityrosine and Hexanoyl-Lysine in Young People: A Pilot Study Int J Environ Res Public Health, 2020-07-10;17(14):. 2020-07-10 [PMID: 32664406] (Human)
• HK Lee, KW Kim, JS Ryu, HJ Jeong, SM Lee, MK Kim Bilateral Effect of the Unilateral Corneal Nerve Cut on Both Ocular Surface and Lacrimal Gland Invest. Ophthalmol. Vis. Sci., 2019-01-02;60(1):430-441. 2019-01-02 [PMID: 30703211] (Mouse)
• Q Li, X Wu, Y Yang, Y Zhang, F He, X Xu, Z Zhang, L Tao, C Luo Tachykinin NK1 receptor antagonist L-733,060 and substance P deletion exert neuroprotection through inhibiting oxidative stress and cell death after traumatic brain injury in mice Int. J. Biochem. Cell Biol., 2018-12-26;0(0):. 2018-12-26 [PMID: 30593954] (Mouse)
• Y Xu, C Huang, H Deng, J Jia, Y Wu, J Yang, W Tu TRPA1 and substance P mediate stress induced duodenal lesions in water immersion restraint stress rat model Turk J Gastroenterol, 2018-11-01;29(6):692-700. 2018-11-01 [PMID: 30381276] (Mouse)
• Li Z, Luo T, Ning X, Xiong C, Wu A Neurokinin-1 receptor antagonism improves postoperative neurocognitive disorder in mice. Neurosci Lett, 2018-09-28;687(0):189-195. 2018-09-28 [PMID: 30273701] (Mouse)
• DSC Butler, I Ambite, K Nagy, C Cafaro, A Ahmed, A Nadeem, N Filenko, TH Tran, KE Andersson, B Wullt, M Puthia, C Svanborg Neuroepithelial control of mucosal inflammation in acute cystitis Sci Rep, 2018-07-20;8(1):11015. 2018-07-20 [PMID: 30030504] (Mouse)
• L Lorente, MM Martín, A Pérez-Ceja, J Ferreres, J Solé-Violá, L Labarta, C Díaz, A Jiménez Sustained Low Serum Substance P Levels in Non-Surviving Septic Patients Int J Mol Sci, 2017-07-15;18(7):. 2017-07-15 [PMID: 28714876] (Human)
• L Xiao, M Ding, A Fernandez, P Zhao, L Jin, X Li Curcumin alleviates lumbar radiculopathy by reducing neuroinflammation, oxidative stress and nociceptive factors Eur Cell Mater, 2017-05-09;33(0):279-293. 2017-05-09 [PMID: 28485773] (Mouse)
• AJ Sandweiss, MI McIntosh, A Moutal, R Davidson-K, J Hu, AK Giri, T Yamamoto, VJ Hruby, R Khanna, TM Largent-Mi, TW Vanderah Genetic and pharmacological antagonism of NK1 receptor prevents opiate abuse potential Mol. Psychiatry, 2017-05-09;0(0):. 2017-05-09 [PMID: 28485408] (Rat)
• Y Okamura, S Mishima, JI Kashiwakur, T Sasaki-Sak, S Toyoshima, K Kuroda, S Saito, Y Tokuhashi, Y Okayama The dual regulation of substance P-mediated inflammation via human synovial mast cells in rheumatoid arthritis Allergol Int, 2017-03-31;0(0):. 2017-03-31 [PMID: 28366675] (Human)
• JA Singh, S Noorbalooc, KL Knutson Cytokine and neuropeptide levels are associated with pain relief in patients with chronically painful total knee arthroplasty: a pilot study BMC Musculoskelet Disord, 2017-01-14;18(1):17. 2017-01-14 [PMID: 28088207] (Human)
• Sphenopalatine Ganglion Acupuncture Improves Nasal Ventilation and Modulates Autonomic Nervous Activity in Healthy Volunteers: A Randomized Controlled Study Sci Rep, 2016-07-18;6(0):29947. 2016-07-18 [PMID: 27425415] (Human)
• M E Olson Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves BMC Vet Res, 2016-06-13;12(1):102. 2016-06-13 [PMID: 27295955] (Bovine)
• A Denes Brain injury induces specific changes in the caecal microbiota of mice via altered autonomic activity and mucoprotein production Brain Behav. Immun., 2016-04-06;57(0):10-20. 2016-04-06 [PMID: 27060191] (Mouse)
• Liu , Boyi, Escalera , Jasmine, Balakrishna , Shrilath, Fan , Lu, Caceres , Ana I, Robinson , Eve, Sui , Aiwei, McKay , M Craig, McAlexander , M Allen, Herrick , Christin, Jordt , Sven E TRPA1 controls inflammation and pruritogen responses in allergic contact dermatitis. FASEB J, 2013-05-30;27(9):3549-63. 2013-05-30 [PMID: 23722916] (Mouse)
• Kempuraj D, Twait E, Williard D, Yuan Z, Meyerholz D, Samuel I The novel cytokine interleukin-33 activates acinar cell proinflammatory pathways and induces acute pancreatic inflammation in mice. PLoS ONE, 2013-02-13;8(2):e56866. 2013-02-13 [PMID: 23418608] (Mouse, Rat)
• Chauhan VS, Kluttz JM Prophylactic and Therapeutic Targeting of the Neurokinin-1 Receptor Limits Neuroinflammation in a Murine Model of Pneumococcal Meningitis. J. Immunol., 2011-05-11;0(0):. 2011-05-11 [PMID: 21562162] (Mouse)
• Lee JK, Song YS, Shin JS, Kwon YS, Shin MS, Kim JC The change of cytokines in tear and blood after different pterygium operation. Cytokine, 2009-12-08;49(2):148-54. 2009-12-08 [PMID: 20004113] (Human)
• Akazawa T, Kwatra SG, Goldsmith LE, Richardson MD, Cox EA, Sampson JH, Kwatra MM A constitutively active form of neurokinin 1 receptor and neurokinin 1 receptor-mediated apoptosis in glioblastomas. J. Neurochem., 2009-03-11;109(4):1079-86. 2009-03-11 [PMID: 19519779] (Human)
• Desouza IA, Camargo EA, Mariano NS, Optiz-Neto JB, Resende JS, Mello GC, Costa SK, De Nucci G, Antunes E Role of sensory innervation in the rat pulmonary neutrophil recruitment induced by staphylococcal enterotoxins type A and B. Eur. J. Pharmacol., 2009-04-16;613(1):128-34. 2009-04-16 [PMID: 19375418] (Rat)
• Templeton A, Nguyen G, Ash JD, Straub RH, Carr DJ Chemical sympathectomy increases susceptibility to ocular herpes simplex virus type 1 infection. J. Neuroimmunol., 2008-05-20;197(1):37-46. 2008-05-20 [PMID: 18495255] (Mouse)
• Dansereau MA, Gosselin RD, Pohl M, Pommier B, Mechighel P, Mauborgne A, Rostene W, Kitabgi P, Beaudet N, Sarret P, Melik-Parsadaniantz S Spinal CCL2 pronociceptive action is no longer effective in CCR2 receptor antagonist-treated rats. J. Neurochem., 2008-04-17;106(2):757-69. 2008-04-17 [PMID: 18419759] (Rat)
• Nina-Li Avellán, Timo Sorsa, Taina Tervahartiala, Clemens Forster, Pentti Kemppainen Experimental tooth pain elevates substance P and matrix metalloproteinase-8 levels in human gingival crevice fluid Acta Odontol Scand 2008-02-04 [PMID: 18320414] (Human)
• Shigematsu N, Fukuda T, Yamamoto T, Nishioku T, Yamaguchi T, Himeno M, Nakayama KI, Tsukuba T, Kadowaki T, Okamoto K, Higuchi S, Yamamoto K Association of cathepsin E deficiency with the increased territorial aggressive response of mice. J. Neurochem., 2008-01-21;105(4):1394-404. 2008-01-21 [PMID: 18221376] (Mouse)
• Im HJ, Li X, Muddasani P, Kim GH, Davis F, Rangan J, Forsyth CB, Ellman M, Thonar EJ Basic fibroblast growth factor accelerates matrix degradation via a neuro-endocrine pathway in human adult articular chondrocytes. J. Cell. Physiol., 2008-05-01;215(2):452-63. 2008-05-01 [PMID: 17960584] (Human)
• Tessier J, Green C, Padgett D, Zhao W, Schwartz L, Hughes M, Hewlett E Contributions of histamine, prostanoids, and neurokinins to edema elicited by edema toxin from Bacillus anthracis. Infect. Immun., 2007-01-29;75(4):1895-903. 2007-01-29 [PMID: 17261611] (Human)
• Bruno MA, Cuello AC Activity-dependent release of precursor nerve growth factor, conversion to mature nerve growth factor, and its degradation by a protease cascade. Proc. Natl. Acad. Sci. U.S.A., 2006-04-17;103(17):6735-40. 2006-04-17 [PMID: 16618925] (Rat)

FAQs for Substance P (KGE007). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.