STRN4 Antibody - BSA Free

Western Blot: STRN4 Antibody [NBP2-36537] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Striatin-4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: STRN4 Antibody [NBP2-36537] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Striatin-4, A304-573A was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

STRN4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit STRN4 Antibody - BSA Free (NBP2-36537) is a polyclonal antibody validated for use in WB and IP. Anti-STRN4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by the antibody maps to a region between residue 325 to 375 of human Striatin-4 using the numbering given in entry NP_037535.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STRN4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:2000 - 1:10000
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STRN4 Antibody - BSA Free

• FLJ35594
• striatin, calmodulin binding protein 4
• striatin-4
• ZIN
• zinedin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STRN4 Antibody (NBP2-36537)(2)

Publications using NBP2-36537

• Cull J, Cooper S, Alharbi H et al. Striatin plays a major role in angiotensin II-induced cardiomyocyte and cardiac hypertrophy in micein vivo bioRxiv 2023-10-25 (Western Blot, Human)
• Joshua J. Cull, Susanna T.E. Cooper, Hajed O. Alharbi, Sonia P. Chothani, Owen J.L. Rackham, Daniel N. Meijles, Philip R. Dash, Risto Kerkelä, Neil Ruparelia, Peter H. Sugden, Angela Clerk Striatin plays a major role in angiotensin II-induced cardiomyocyte and cardiac hypertrophy in mice in vivo Clinical Science (London, England : 1979) 2024-05-01 [PMID: 38718356]

Bioinformatics

• Gene Symbol: STRN4
• Uniprot:
  • NP_037535.2(Human)