SG2NA Antibody (S68)

Western Blot: SG2NA Antibody (S68) [NB110-74572] - Detection of SG2NA in NIH3T3 cell lysates using NB110-74572.

Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SG2NA (S68) [NB110-74572] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: SG2NA Antibody (S68) [NB110-74572] - Immunofluorescent staining of NIH3T3 cells using NB110-74572.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: S68
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SG2NA Antibody (S68) Summary

• Immunogen: Synthetic peptide made to an internal portion of human SG2NA (within residues 200-300). [Swiss-Prot# Q13033]
• Localization: Cytoplasm and membrane.
• Specificity: This antibody is specific for SG2NA. It does not cross-react with Striatin.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STRN3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:250-1:2000
• Application Notes: This SG2NA antibody is useful in Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at ~90 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for SG2NA Antibody (S68)

• Cell cycle autoantigen SG2NA
• cell cycle S/G2 nuclear autoantigen
• GS2NA
• S/G2 antigen
• SG2NAnuclear autoantigen
• striatin, calmodulin binding protein 3
• striatin-3

Background

SG2NA was identified as a human cancer autoantigen and has now been implicated in vesicular trafficking and signal transduction. It is highly related to striatin and zinedin. It is a scaffolding protein that binds PP2A A and C subunits as well as the human homolog of the yeast protein, Mob1, which has been implicated in control of mitotic progression in yeast. It also binds calmodulin and appears to modulate PP2A activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SG2NA Antibody (NB110-74572)(6)

Publications using NB110-74572

• Juraszek B, Natcz KA. Protein phosphatase PP2A - a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes. J. Neurochem. 2016-08-18 [PMID: 27537937] (ICC/IF, WB)
• Tanti GK, Goswami SK. SG2NA recruits DJ-1 and Akt into the mitochondria and membrane to protect cells from oxidative damage. Free Radic. Biol. Med. 2014-07-15 [PMID: 25035075] (ICC/IF, IP, WB, Mouse)
  
  Details:
  SG2NA antibody used for WB, IP and ICC-IF. WB performed in several experiments involving NIH3T3 cells, Neuro2A cells and mouse brain. IP performed on Neuro2A cells and Mouse brain. ICC-IF performed on Neuro2A cells - fixed in 3.7% PFA/10% FBS media for 15 min, permeabilized with 0.2% Triton X-100 in 1X PBS for 15 min, primary used at 1:100 dilution with 4C ON incubation. WB and ICC-IF also performed on Neuro2A cells depleted from SG2NA with SG2NA-shRNA constructs.
• Tran H, Bustos D, Yeh R et al. HectD1 E3 Ligase Modifies Adenomatous Polyposis Coli (APC) with Polyubiquitin to Promote the APC-Axin Interaction. J Biol Chem 2012-12-31 [PMID: 23277359] (WB, Human)
• Moreno, CS et al. A mammalian homolog of yeast MOB1 is both a member and a putative substrate of striatin family-protein phosphatase 2A complexes. J Biol Chem;276(26):24253-60. 2001-06-29 [PMID: 11319234] (ICC/IF, IP, Human, Mouse)
• Yu, XX et al. Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Balpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen. Mol Biol Cell;12(1):185-99. 2001-01-01 [PMID: 11160832] (IP)
• Moreno, CS et al. WD40 repeat proteins striatin and S/G(2) nuclear autoantigen are members of a novel family of calmodulin-binding proteins that associate with protein phosphatase 2A. J Biol Chem;275(8):5257-63. 2000-02-25 [PMID: 10681496] (IP, Human)

Reviews for SG2NA Antibody (NB110-74572) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer Proximity Ligation Assay (PLA) Rat 01/25/2017

Summary

• Application: Other
• Sample Tested: Paraformeldehyde Fixed Cells
• Species: Rat
• Lot: B1

reviewed by: Cynthia Lam WB Human 05/11/2016

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human

reviewed by: Verified Customer WB Human 05/11/2016

Summary

• Application: Western Blot
• Sample Tested: 293T lysate
• Species: Human

FAQs for SG2NA Antibody (NB110-74572). (Showing 1 - 1 of 1 FAQs).

1. What is the concentration of this antibody?
   • Unfortunately, we have not determined the concentration for this antibody. Please note that none of unpurified antibodies derived directly from ascites is subjected to the concentration determination. This is because there are so many IgG-unrelated proteins that are present in ascites, and the readings could reach to the double-digit in mg/ml. Therefore the usefulness of this kind of antibodies are indicated as the dilution factors for the different applications, after lab-testings

Bioinformatics

• Gene Symbol: STRN3
• Entrez:
  • 29966(Human)
  • 94186(Mouse)
  • 114520(Rat)
• Uniprot:
  • Q13033(Human)