STRA8 Antibody (PSH04-55)

Western Blot: STRA8 Antibody (PSH04-55) [NBP3-32983] - Western blot analysis of STRA8 on different lysates with Rabbit anti-STRA8 antibody (NBP3-32983) at 1/1,000 dilution. Lane 1: SK-OV-3 cell lysate Lane 2: LNCaP cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 37 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32983) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: STRA8 Antibody (PSH04-55) [NBP3-32983] - Immunocytochemistry analysis of SK-OV-3 cells labeling STRA8 with Rabbit anti-STRA8 antibody (NBP3-32983) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STRA8 antibody (NBP3-32983) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: STRA8 Antibody (PSH04-55) [NBP3-32983] - Flow cytometric analysis of SK-OV-3 cells labeling STRA8. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32983, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: PSH04-55
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

STRA8 Antibody (PSH04-55) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human STRA8 aa 1-330 / 330. (Uniprot: Q7Z7C7)
• Localization: Cytoplasm, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STRA8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Western Blot 1:1000
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for STRA8 Antibody (PSH04-55)

• stimulated by retinoic acid gene 8 homolog (mouse)
• stimulated by retinoic acid gene 8 protein homolog

Background

STRA8 is required for the transition into meiosis for both female and male germ cells. In female germ cells, requiredfor premeiotic DNA replication and subsequent events in meiotic prophase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STRA8