STIM1 Antibody - Azide Free

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human thyroid tissue

Immunohistochemistry-Paraffin: STIM1 Antibody [NBP2-44168] - Staining of human testis tissue.

Immunohistochemistry of Sheep STIM1 Antibody. Tissue: Thyroid. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Sheep STIM1 Antibody. Tissue: Testis. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: GOK / STIM1 antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase sheep secondary antibody at 1:10,000 for 45 min at RT. Staining: GOK / STIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: Azide Free

STIM1 Antibody - Azide Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: STIM1 Antibody was prepared from whole sheep serum produced by repeated immunizations with a synthetic peptide near the N-terminus of human STIM1. (Uniprot: Q13586)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STIM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 5ug/ml
  • Immunohistochemistry-Paraffin
  • Western Blot 1ug/ml
• Application Notes: This product is tested for ELISA and immunohistochemistry and suitable for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest cross-reactivity with rat, human, and mouse based on 100% sequence homology. Cross-reactivity with Stim I from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (BSA)
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for STIM1 Antibody - Azide Free

• GOK
• GOKD11S4896E
• STIM1
• stromal interaction molecule 1

Background

Stromal interaction molecule 1 plays a role in mediating Ca(2+) influx following depletion of intracellular Ca(2+) stores. It acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, it translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STIM1
• Uniprot:
  • Q13586()
  • Q13586()
  • Q13586()
  • Q13586()