STIM1 Antibody (705111) [Alexa Fluor® 594]

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clone: 705111
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

STIM1 Antibody (705111) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human STIM1
  Leu23-Thr182
  Accession # Q13586
• Specificity: Detects human STIM1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human STIM2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STIM1 Antibody (705111) [Alexa Fluor® 594]

• GOK
• GOKD11S4896E
• STIM1
• stromal interaction molecule 1

Background

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2+ release-activated Ca2+ (CRAC) channel Orai1, increasing Ca2+ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2+-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.