STI1 Antibody

Images

 
Western Blot: STIP1 Antibody [NBP2-20512] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: STIP1 Antibody [NBP2-20512] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STIP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: STIP1 Antibody [NBP2-20512] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.89 mg/ml

Order Details

STI1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.
Localization
Cytoplasm (By similarity); Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STIP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.89 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STI1 Antibody

  • HOP
  • HOPSTI1L
  • Hsc70/Hsp90-organizing protein
  • Hsp70/Hsp90-organizing protein
  • IEF-SSP-3521
  • NY-REN-11 antigen
  • NY-REN-11
  • Renal carcinoma antigen NY-REN-11
  • STI1
  • STI1L
  • STI1P60
  • STIP1
  • stress-induced-phosphoprotein 1
  • Transformation-sensitive protein IEF SSP 3521

Background

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for STI1 Antibody (NBP2-20512) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STIP1
Entrez
Uniprot