STI1 Antibody

Western blot shows lysates of U2OS human osteosarcoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Mouse/Rat STI1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7337) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for STI1 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

STI1 Antibody Summary

• Immunogen: E. coli-derived recombinant human STI1
  Met1-Arg543
  Accession # P31948
• Specificity: Detects human, mouse, rat STI1 in Western blots and detects recombinant human STI1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STIP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STI1 Antibody

• HOP
• HOPSTI1L
• Hsc70/Hsp90-organizing protein
• Hsp70/Hsp90-organizing protein
• IEF-SSP-3521
• NY-REN-11 antigen
• NY-REN-11
• Renal carcinoma antigen NY-REN-11
• STI1
• STI1L
• STI1P60
• STIP1
• stress-induced-phosphoprotein 1
• Transformation-sensitive protein IEF SSP 3521

Background

STI1 (Stress-induced phosphoprotein 1; also HOP and STIP-1) is a 60-62 kDa member of the STI-1 family of proteins. It is widely expressed, and participates in the formation of functional steroid hormone receptor complexes. Steroid receptors (SR) require proper folding to achieve optimal conformation. In the case of progesterone and glucocorticoid receptors, interaction with chaperones hsp70 and hsp90 facilitates this folding. STI1 is not a chaperone, but a mediator of SR-hsp70-hsp90 interaction. STI1 first binds dimeric hsp90. This trimer then interacts with a SR-(monomeric)hsp70-ADP protein heterodimer to form a SR-hsp70-STI1-hsp90 pentamer that subsequently dissociates into a mature hsp90-SR complex. The STI1-hsp90 complex is also found extracellularly and may contribute to MMP-2 activation. Human STI1 is 543 amino acids (aa) in length. It contains an initial N-terminal TPR domain (aa 4-105) that binds to hsp70, followed by a second TPR domain (aa 225-461) that binds to dimeric hsp90. There are at least 10 acetylated Lys residues plus four utilized phosphorylation sites. Zone potential isoform variant is reported that contains a 48 aa substitution for Gln3. Full-length human STI1 shares 97% aa sequence identity with mouse STI1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STI1 Antibody (AF7337)(1)

Publication using AF7337

• VD Atayde, A da Silva L, V Chaparro, A Zimmermann, C Martel, M Jaramillo, M Olivier Exploitation of the Leishmania exosomal pathway by Leishmania RNA virus 1 Nat Microbiol, 2019-01-28;0(0):. 2019-01-28 [PMID: 30692670] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: STIP1
• Uniprot:
  • P31948(Human)