STAT6 Antibody [Unconjugated]

Western blot shows lysates of TF‑1 human erythroleukemic cell line and human peripheral blood lymphocyte. PVDF membrane was probed with 1:2000 dilution of Human/Mouse STAT6 Polyclonal Antibody (Catalog # PA-ST6) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for STAT6 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

STAT6 Antibody [Unconjugated] Summary

• Specificity: Detects human and murine STAT 6. This antiserum does not crossreact with any of the other STAT proteins.
• Source: N/A
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT6

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 3-6 ug/10^6 cells
  • Western Blot 2000x ug/mL

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Lyophilized from 0.2 mL antiserum containing 0.02% sodium azide
• Preservative: 0.02% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT6 Antibody [Unconjugated]

• D12S1644
• EC 2.4.1.227
• EC 2.7.7.6
• IL-4 Stat
• IL-4-STAT
• signal transducer and activator of transcription 6
• signal transducer and activator of transcription 6, interleukin-4 induced
• STAT, interleukin-4 induced
• STAT, interleukin4-induced
• STAT6
• STAT6B
• STAT6C
• transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Jak/STAT signal transduction pathway and mediates cytokine signaling by IL-4. IL-4 activation leads to tyrosine phosphorylation and accumulation in the nucleus of STAT6 homo and heterodimers. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN₄GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. STAT6 transcriptional activation occurs by binding p100 and p160 class (NcoA1, Src-1) co-activators. Gene expression studies indicate that STAT6 mediates cell proliferation and anti-apoptotic effects of IL-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT6 Antibody (PA-ST6)(1)

Publication using PA-ST6

• O'Connor JC, Sherry CL, Guest CB, Freund GG Type 2 diabetes impairs insulin receptor substrate-2-mediated phosphatidylinositol 3-kinase activity in primary macrophages to induce a state of cytokine resistance to IL-4 in association with overexpression of suppressor of cytokine signaling-3. J. Immunol., 2007-06-01;178(11):6886-93. 2007-06-01 [PMID: 17513737] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: STAT6