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STAT6 Antibody [HRP]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB600-229AF350
Alexa Fluor 405
NB600-229AF405
Alexa Fluor 488
NB600-229AF488
Alexa Fluor 532
NB600-229AF532
Alexa Fluor 594
NB600-229AF594
Alexa Fluor 647
NB600-229AF647
Alexa Fluor 700
NB600-229AF700
Alexa Fluor 750
NB600-229AF750
Biotin
NB600-229B
DyLight 350
NB600-229UV
DyLight 405
NB600-229V
DyLight 488
NB600-229G
DyLight 550
NB600-229R
DyLight 594
NB600-229DL594
DyLight 650
NB600-229C
DyLight 680
NB600-229FR
DyLight 755
NB600-229IR
FITC
NB600-229F
Janelia Fluor 549
NB600-229JF549
Janelia Fluor 646
NB600-229JF646
Unconjugated
NB600-229
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STAT6 Antibody [HRP] Summary

Immunogen
The immunogen recognized by the STAT6 Antibody maps to a region between residue 600 and 650 of human Signal Transducer and Activator of Transcription 6 (NP_003144.3).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STAT6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
STAT6 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for STAT6 Antibody [HRP]

  • D12S1644
  • EC 2.4.1.227
  • EC 2.7.7.6
  • IL-4 Stat
  • IL-4-STAT
  • signal transducer and activator of transcription 6
  • signal transducer and activator of transcription 6, interleukin-4 induced
  • STAT, interleukin-4 induced
  • STAT, interleukin4-induced
  • STAT6
  • STAT6B
  • STAT6C
  • transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22471
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IgE Secondary Antibod
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Western Blot: Jak3 Antibody (5H2) [NBP2-37737] - Western blot analysis using JAK3 mouse mAb against Jurkat cell lysate (1).
Immunocytochemistry/Immunofluorescence: Jak3 Antibody (5H2) [NBP2-37737] - Confocal immunofluorescence analysis of 3T3-L1 cells using JAK3 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: Jak3 Antibody (5H2) [NBP2-37737] - Flow cytometric analysis of Hela cells using JAK3 mouse mAb (blue) and negative control (red).
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Publications for STAT6 Antibody (NB600-229H) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAT6 Antibody (NB600-229H) (0)

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Control Lysate(s)

STAT6 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-229AF350
Alexa Fluor 405 NB600-229AF405
Alexa Fluor 488 NB600-229AF488
Alexa Fluor 532 NB600-229AF532
Alexa Fluor 594 NB600-229AF594
Alexa Fluor 647 NB600-229AF647
Alexa Fluor 700 NB600-229AF700
Alexa Fluor 750 NB600-229AF750
Biotin NB600-229B
DyLight 350 NB600-229UV
DyLight 405 NB600-229V
DyLight 488 NB600-229G
DyLight 550 NB600-229R
DyLight 594 NB600-229DL594
DyLight 650 NB600-229C
DyLight 680 NB600-229FR
DyLight 755 NB600-229IR
FITC NB600-229F
HRP NB600-229H
Janelia Fluor 549 NB600-229JF549
Janelia Fluor 646 NB600-229JF646
Learn about Custom Antibody Labeling

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Bioinformatics Tool for STAT6 Antibody (NB600-229H)

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Blogs on STAT6.

Check out the latest blog posts on STAT6.
Signal Transducer and Activator of Transcription STAT6: More than a Player in Allergic Inflammation
By Jamshed Arslan, Pharm. D., PhD. What is STAT6?The cellular pathway comprising tyrosine kinase Janus Kinase (JAK) and the transcription factor STAT connect extracellular signals from various cytokines, hormones an...  Read full blog post.
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Bioinformatics

Gene Symbol STAT6
Entrez