STAT6 Antibody (6C10) - Azide and BSA Free

Western Blot: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of STAT6 monoclonal antibody (M01, clone 6C10) expression in HeLa. Band was detected at a molecular weight of approximately 94 kDa.

Immunocytochemistry/Immunofluorescence: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of monoclonal antibody to STAT6 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of monoclonal antibody to STAT6 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.

Western Blot: STAT6 Antibody (6C10) [H00006778-M01] - Analysis of STAT6 expression in transfected 293T cell line by STAT6 monoclonal antibody (M01, clone 6C10). Lane 1: STAT6 transfected lysate (Molecular Weight of approximately 94 KDa). Lane 2: Non-transfected lysate.

Sandwich ELISA: STAT6 Antibody (6C10) [H00006778-M01] - Detection limit for recombinant GST tagged STAT6 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 6C10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STAT6 Antibody (6C10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: STAT6 (NP_003144, 694 a.a. ~ 801 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPHSHSIPPYQGLSPEESVNVLSAFQEPHLQMPPSLGQMSLPFDQPHPQGLLPCQPQEHAVSSPDPLLCSDVTMVEDSCLSQPVTAFPQGTWIGEDIFPPLLPPTEQD
• Specificity: STAT6 (6C10)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STAT6 Antibody (6C10) - Azide and BSA Free

• D12S1644
• EC 2.4.1.227
• EC 2.7.7.6
• IL-4 Stat
• IL-4-STAT
• signal transducer and activator of transcription 6
• signal transducer and activator of transcription 6, interleukin-4 induced
• STAT, interleukin-4 induced
• STAT, interleukin4-induced
• STAT6
• STAT6B
• STAT6C
• transcription factor IL-4 STAT

Background

Signal Transducer and Activator of Transcription 6 (STAT6) is a member of the Janus family tyrosine kinases (Jak)/ STAT signal transduction pathway and mediates cytokine signaling by IL 4 and IL-13 (1). STAT6 (predicted molecular weight 94kDa) has been found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL-4. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT6 is tyrosine phosphorylated (Tyr641) in response to cellular interaction with IL-4. STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta. STAT6 is critically involved in Th2 and Th9 immune response (2). Loss of STAT6 impairs IL-4 mediated functions including Th2 helper T cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation. Diseases associated with STAT6 include malignant hemangiopericytoma and nut allergies.

References

1. Waqas, S. F. H., Ampem, G., & Roszer, T. (2019). Analysis of IL-4/STAT6 Signaling in Macrophages. Methods Mol Biol, 1966, 211-224. doi:10.1007/978-1-4939-9195-2_17

2. Goenka, S., & Kaplan, M. H. (2011). Transcriptional regulation by STAT6. Immunol Res, 50(1), 87-96. doi:10.1007/s12026-011-8205-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT6 Antibody (H00006778-M01)(1)

Publication using H00006778-M01

• Slawinska M, Lakomy J, Biernat W et al. STAT3, STAT5A, STAT5B, STAT6 proteins are overexpressed in human basal cell carcinoma Clin. Exp. Dermatol. 2019-07-19 [PMID: 31323143] (IF/IHC, Human)

FAQs for STAT6 Antibody (H00006778-M01). (Showing 1 - 1 of 1 FAQ).

1. For antibodies : STAT5b H00006777-M03; STAT 5a NBR2-00622; STAT3 NBP2-22471; STAT 6 H00006778-M01 - do these detect phosphorylated , unphosphorylated or both forms of STATs?
   • Antibodies H00006777-M03, NBR2-00622,  NBP2-22471,  and H00006778-M01 were raised to recognize total STAT proteins, they do not differentiate between any posttranslational modifications and will recognize both phosphorylated and non-phosphorylated protein. 

Bioinformatics

• Gene Symbol: STAT6
• Entrez:
  • 6778(Human)
• OMIM:
  • 601512(Human)
• Uniprot:
  • NP_003144()