STAT4 Overexpression Lysate (Native)

Images

 
Western Blot: STAT4 Overexpression Lysate (Adult Normal) [NBL1-16527] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for STAT4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

STAT4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206892 and based on accession number NM_003151. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens signal transducer and activator of transcription 4 (STAT4), mRNA.
Gene
STAT4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
85.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for STAT4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for STAT4 Overexpression Lysate (Native)

  • signal transducer and activator of transcription 4
  • SLEB11
  • STAT4

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for STAT4 Lysate (NBL1-16527) (0)

There are no publications for STAT4 Lysate (NBL1-16527).
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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol STAT4
Entrez