STAT2 Antibody (4A5P8)

Western Blot: STAT2 Antibody (4A5P8) [NBP3-16228] - Western blot analysis of lysates from wild type (WT) and STAT2 knockdown (KD) 293T cells, using [KD Validated] STAT2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

Western Blot: STAT2 Antibody (4A5P8) [NBP3-16228] - Western blot analysis of various lysates using [KD Validated] STAT2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.

Western Blot: STAT2 Antibody (4A5P8) [NBP3-16228] - Western blot analysis of lysates from Rat liver, using [KD Validated] STAT2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Rt
• Applications: WB
• Clone: 4A5P8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

STAT2 Antibody (4A5P8) Summary

• Description: Novus Biologicals Rabbit STAT2 Antibody (4A5P8) (NBP3-16228) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 550-706 of human STAT2 (NP_005410.1). KLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDEL
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: STAT2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for STAT2 Antibody (4A5P8)

• interferon alpha induced transcriptional activator
• ISGF-3
• MGC59816
• P113
• signal transducer and activator of transcription 2
• signal transducer and activator of transcription 2, 113kD
• signal transducer and activator of transcription 2, 113kDa
• STAT113
• STAT2

Background

Stat-2 is a latent transcription factor that functions both as a signal transducer in the cytoplasm and an activator of transcription in the nucleus. Stat-2 is one of seven members of the Signal transducer and activators of transcription (Stats) family. Stat-2 is phosphorylated at Tyr690 by JAKs(1-3). Additionally, upon activation, Stat-2 will form a multiprotein DNA-binding complex with Stat-1 and ISGF3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT2