STAT1 Antibody (SM1) - BSA Free

Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT1 (Ser727) by the antibody PSM1 (upper panel) and total STAT1 level by the antibody SM1 (lower panel).

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clone: SM1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

STAT1 Antibody (SM1) - BSA Free Summary

• Immunogen: STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
• Specificity: The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (paraffin sections) positive tissue: lung macrophages.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for STAT1 Antibody (SM1) - BSA Free

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT1 Antibody (NB500-514)(2)

Publications using NB500-514

• Boudny V, Kocak I, Lauerova L, Kovarik J. Interferon inducibility of STAT 1 activation and its prognostic significance in melanoma patients. Folia Biol (Praha);49(4):142-6. 2003-01-01 [PMID: 12971583]
• Kovarik J, Boudny V, Kocak I et al. Malignant melanoma associates with deficient IFN-induced STAT 1 phosphorylation. Int J Mol Med;12(3):335-40. 2003-09-01 [PMID: 12883649]

Bioinformatics

• Gene Symbol: STAT1
• Entrez:
  • 6772(Human)
  • 20846(Mouse)
• Uniprot:
  • P42224()