STAT1 Antibody (SM1)

Images

 
Immunoprecipitation: STAT1 Antibody (SM1) [NB500-514] - Fig. 1: Induction of phosphorylation of STAT1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
SM1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

STAT1 Antibody (SM1) Summary

Immunogen
STAT1 peptide sequence 721-733 (DNLLPMSPEEFDE).
Specificity
The antibody SM1 recognizes an epitope included within amino acids 721-733 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
STAT1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:50
Publications
Read Publications using NB500-514.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for STAT1 Antibody (SM1)

  • CANDF7
  • DKFZp686B04100
  • ISGF-3
  • ISGF-3,91kD
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription-1
  • STAT1
  • STAT91

Background

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
NB400-141
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
AF2988
Species: Mu, Rt
Applications: WB
MAB4260
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
NBP2-00622
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow
AF2890
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
AF4715
Species: Mu
Applications: WB
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF5629
Species: Hu, Mu
Applications: WB

Publications for STAT1 Antibody (NB500-514)(2)

Reviews for STAT1 Antibody (NB500-514) (0)

There are no reviews for STAT1 Antibody (NB500-514). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for STAT1 Antibody (NB500-514) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional STAT1 Products

Bioinformatics Tool for STAT1 Antibody (NB500-514)

Discover related pathways, diseases and genes to STAT1 Antibody (NB500-514). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for STAT1 Antibody (NB500-514)

Discover more about diseases related to STAT1 Antibody (NB500-514).
 

Pathways for STAT1 Antibody (NB500-514)

View related products by pathway.

PTMs for STAT1 Antibody (NB500-514)

Learn more about PTMs related to STAT1 Antibody (NB500-514).
 

Research Areas for STAT1 Antibody (NB500-514)

Find related products by research area.

Blogs on STAT1.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our STAT1 Antibody (SM1) and receive a gift card or discount.

Bioinformatics

Gene Symbol STAT1
Entrez
Uniprot