STAT1 [p Ser727] Antibody (PSM1) - BSA Free

Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.

• Reactivity: Hu
• Applications: WB, IP
• Clone: PSM1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

STAT1 [p Ser727] Antibody (PSM1) - BSA Free Summary

• Description: Novus Biologicals Mouse STAT1 [p Ser727] Antibody (PSM1) - BSA Free (NB500-515) is a monoclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: STAT1 peptide sequence 721-733 (Ser727 phosphorylated).
• Modification: p Ser727
• Specificity: The antibody PSM1 recognizes transcriptional factor STAT1 (91 kDa) activated by phosphorylation at Ser727.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: WB: Detects a band of approximately 91 kDa. Purity is > 95% (by SDS-PAGE).
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for STAT1 [p Ser727] Antibody (PSM1) - BSA Free

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STAT1 (signal transducer and activator of transcription 1) is a transcription factor that plays important roles in growth arrest, apoptosis promoting and tumour suppression. After ligation of cytokine receptors STAT1 becomes phosphorylated on Tyr701 by Janus kinase JAK1 or JAK2, dimerizes, translocates to nucleus and contacts DNA. STAT1-STAT2 heterodimers serve as more potent transcriptional inducers than STAT1 homodimers. STAT1 is also phosphorylated on Ser727 by MAPK pathway, independently of tyrosine phosphorylation. However, the both modifications are important for its maximal transcriptional activity. On the other hand, STAT1 phosphorylated on Ser727 is targeted for proteasomal degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT1
• Entrez:
  • 6772(Human)
• Uniprot:
  • P42224()