STAT1 Antibody (OTI4H9)

Western Blot: STAT1 Antibody (OTI4H9) [NBP1-48011] - Analysis of extracts (10ug) from 7 Human tissue by using anti-STAT1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.

Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY STAT1.

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human Kidney tissue using anti-STAT1 mouse monoclonal antibody.

Western Blot: STAT1 Antibody (4H9) [NBP1-48011] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT1.

Western Blot: STAT1 Antibody (4H9) [NBP1-48011] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-STAT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: STAT1 Antibody (4H9) [NBP1-48011] - Immunofluorescent staining of HT29 cells using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human breast tissue using anti-STAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: STAT1 Antibody (4H9) [NBP1-48011] - Staining of paraffin-embedded Human endometrium tissue using anti-STAT1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI4H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.7 mg/ml

STAT1 Antibody (OTI4H9) Summary

• Description: Novus Biologicals Mouse STAT1 Antibody (OTI4H9) (NBP1-48011) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.
• Specificity: This antibody is specific for Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: STAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
• Theoretical MW: 82.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.7 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for STAT1 Antibody (OTI4H9)

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors,STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for STAT1 Antibody (NBP1-48011) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: rajesha rupaimoole WB Human 10/06/2014

Summary

• Application: Western Blot
• Sample Tested: Human ovarian cancer cell line A2780
• Species: Human

FAQs for STAT1 Antibody (NBP1-48011). (Showing 1 - 1 of 1 FAQ).

1. We are looking for an NBS mouse monoclonal antibody that would work for IF. NBS1 Antibody (NB100-221) might be ideal, except we need to use it in mouse cells, and the data sheet says it is reactive with human cells. Could you tell us if it has been tested in mouse cells? If it has not, we would be willing to test it, and send you verifying images. However, if we test it and it does not work in mouse cells, would we be able to receive another antibody in exchange? We would also like to purchase a STAT1 mouse monoclonal antibody that would work for IF. STAT1 Antibody (NBP1-48011). The species reactivity of the antibody indicates that it is reactive with human, canine, and primate cells. Has it been tested in mouse cells?
   • I have a couple of thoughts after reading through your needs for this antibody. This antibody is validated for mouse reactivity. I assume you realize that if you want to use a mouse mAb on mouse tissue, you will need to do a mouse on mouse block to prevent non-specific staining with your secondary. If you would like to test an antibody in an unvalidated species or application, please consider our Innovators Reward Program. STAT1, NBP1-48011, has not been validated for mouse reactivity so this would qualify for the Innovators Reward Program.

Bioinformatics

• Gene Symbol: STAT1
• Entrez:
  • 6772(Human)