ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Gal Sialyltransferase 1/ST6GAL1
  Lys27-Cys406
  Accession # P15907
• Specificity: Detects human Gal Sialyltransferase 1/ST6GAL1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ST6GAL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Biotin]

• Alpha 2,6-ST
• B-cell antigen CD75
• beta-galactoside alpha-2,6-sialyltransferase 1
• CD75
• CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
• EC 2.4.99.1
• MGC48859
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
• Sialyltransferase 1
• SIAT1
• SIAT1Alpha 2,6-ST 1
• ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• ST6Gal I
• ST6GAL1
• ST6GalI
• ST6N

Background

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
9. Kitazume, S. et al. (2009) Glycobiology 19:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ST6GAL1
• Uniprot:
  • P15907()