Src [p Tyr530] Antibody (S03-8H8)

Western Blot: Src [p Tyr530] Antibody (S03-8H8) [NBP3-20066] - Western blot detection of Src (phospho-Tyr529) in CHO-K1 cell lysates using NBP3-20066 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S03-8H8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Src [p Tyr530] Antibody (S03-8H8) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src (Uniprot # P12931)
• Modification: p Tyr530
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SRC
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Src [p Tyr530] Antibody (S03-8H8)

• ASV
• c-Src
• EC 2.7.10
• EC 2.7.10.2
• pp60c-src
• Rous sarcoma
• RSVgp4
• Src
• tyrosine kinase pp60c-src
• tyrosine-protein kinase SRC-1
• v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a protein tyrosine kinase known to regulate cellular adhesion. Composed of three major domains; SH2, SH3 and a kinase catalytic domain, Src can be switched from an inactive to an active state though control of its phosphorylation state or through protein-protein interaction. Phosphorylation of Y529 by Csk and Chk inactivates Src, while dephosphorylation of Y529 will modify its conformation to an open active state (1-3). Mutation of Y529 leads to Scr overactivity. Several cancers including colon and breast cancer have been associated with an increase of Src activity (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRC