SPRY1 Antibody

Images

 
Western Blot: SPRY1 Antibody [NBP1-51918] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Flow Cytometry: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more
Immunocytochemistry/ Immunofluorescence: SPRY1 Antibody [NBP1-51918] - analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SPRY1 Antibody Summary

Immunogen
Peptide with sequence CPSRGQGKPS corresponding to C-Terminus according to NP_005832.1, NP_955359.1.
Epitope
CPSRGQGKPS
Specificity
NP_005832.1 and NP_955359.1 represent varients of the same protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SPRY1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1 - 3 ug/ml
  • Peptide ELISA detection limit 1:16000
Application Notes
WB successful usage in ERMS cells is reported in scientific literature (PMID: 20068162).
Western blot: Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162).
IHC: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells.
Flow Cytometry: Flow cytometric analysis of HEK293 cells.
Publications
Read Publication using NBP1-51918.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

NP_005832.1 and NP_955359.1 represent varients of the same protein.

Alternate Names for SPRY1 Antibody

  • Drosophila, homolog of, 1 (antagonist of FGF signaling)
  • sprouty homolog 1, antagonist of FGF signaling (Drosophila)
  • SPRY1
  • spry-1

Background

Sprouty may function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPRY1 Antibody (NBP1-51918)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPRY1
Entrez
Uniprot