SPRY1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and LNCaP human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SPRY1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6097) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SPRY1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SPRY1
  Met1-Lys178
  Accession # O43609
• Specificity: Detects human SPRY1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SPRY1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPRY1 Antibody [Unconjugated]

• Drosophila, homolog of, 1 (antagonist of FGF signaling)
• sprouty homolog 1, antagonist of FGF signaling (Drosophila)
• SPRY1
• spry-1

Background

SPRY1 (sprouty homolog 1) is a 34-38 kDa member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gamma 1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 amino acids (aa) in length. It contains one CBL-TKB binding site (aa 51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa 112-131), and a Cys-rich domain (aa 181-306) that mediates intracellular translocation. SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Over aa 1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPRY1
• Uniprot:
  • O43609(Human)