SPRED2 Antibody

Images

 
Western Blot: SPRED2 Antibody [NBP2-20478] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: SPRED2 Antibody [NBP2-20478] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.61 mg/ml

Order Details

SPRED2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SPRED2. The exact sequence is proprietary.
Localization
Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential); Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPRED2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.61 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPRED2 Antibody

  • FLJ31917
  • MGC163164
  • SPRED2
  • Spred-2related sequence
  • sprouty-related, EVH1 domain containing 2
  • sprouty-related, EVH1 domain-containing protein 2

Background

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPRED2
Entrez
Uniprot