SPRED2 Antibody - BSA Free

Western Blot: SPRED2 Antibody [NBP2-20478] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: SPRED2 Antibody [NBP2-20478] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPRED2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPRED2 Antibody - BSA Free (NBP2-20478) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SPRED2. The exact sequence is proprietary.
• Localization: Membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasmic vesicle; secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential); Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPRED2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Macaque.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SPRED2 Antibody - BSA Free

• FLJ31917
• MGC163164
• SPRED2
• Spred-2related sequence
• sprouty-related, EVH1 domain containing 2
• sprouty-related, EVH1 domain-containing protein 2

Background

SPRED2 is a member of the Sprouty (see SPRY1; MIM 602465)/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade (see MAPK1; MIM 176948) (Nonami et al., 2004 [PubMed 15465815]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SPRED2
• Uniprot:
  • Q7Z698()