![Western Blot: SPRED3 Antibody [NBP2-41091] - Analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 ug/mL.](http://images.novusbio.com/fullsize/SPRED3-Antibody-Western-Blot-NBP2-41091-img0004.jpg "Western Blot: SPRED3 Antibody [NBP2-41091] - Analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 ug/mL.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit SPRED3 Antibody - BSA Free (NBP2-41091) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3. The immunogen is located within amino acids 140 - 190 of Spred3. Amino Acid Squence: SHSRQETPPSAAAAPI |
| Specificity | This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SPRED3 |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for SPRED3 Antibody (NBP2-41091)Find related products by research area.
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| Gene Symbol | SPRED3 |
![Immunohistochemistry: SPRED3 Antibody - BSA Free [NBP2-41091] - Immunohistochemistry of SPRED3 in human brain tissue with SPRED3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41091_rabbit-polyclonal-spred3-antibody-271120242074632.jpg "Immunohistochemistry: SPRED3 Antibody - BSA Free [NBP2-41091] - Immunohistochemistry of SPRED3 in human brain tissue with SPRED3 antibody at 2.5 ug/mL.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")