SPRED2 Antibody - BSA Free

Western Blot: SPRED2 Antibody [NBP1-76342] - Human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SPRED2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPRED2 Antibody - BSA Free (NBP1-76342) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2. The immunogen is located within amino acids 140 - 190 of Spred2. Amino Acid Squence: SNSSQKREQPTRT
• Specificity: This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPRED2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1-2 ug/ml
• Control Peptide:

  SPRED2 Peptide (NBP1-76342PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SPRED2 Antibody - BSA Free

• FLJ31917
• MGC163164
• SPRED2
• Spred-2related sequence
• sprouty-related, EVH1 domain containing 2
• sprouty-related, EVH1 domain-containing protein 2

Background

Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist. This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPRED2
• Uniprot:
  • NP_861449(Human)