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Recombinant Human SPRED1 Protein

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SDS-Page: SPRED1 Recombinant Protein [NBP2-51864] - 15% SDS Page (3 ug)
SDS-Page: SPRED1 Recombinant Protein [NBP2-51864] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human SPRED1 Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-444 of Human SPRED1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSEETAT SDNDNSYARV RAVVMTRDDS SGGWLPLGGS GLSSVTVFKV PHQEENGCAD FFIRGERLRD KMVVLECMLK KDLIYNKVTP TFHHWKIDDK KFGLTFQSPA DARAFDRGIR RAIEDISQGC PESKNEAEGA DDLQANEEDS SSSLVKDHLF QQETVVTSEP YRSSNIRPSP FEDLNARRVY MQSQANQITF GQPGLDIQSR SMEYVQRQIS KECGSLKSQN RVPLKSIRHV SFQDEDEIVR INPRDILIRR YADYRHPDMW KNDLERDDAD SSIQFSKPDS KKSDYLYSCG DETKLSSPKD SVVFKTQPSS LKIKKSKRRK EDGERSRCVY CQERFNHEEN VRGKCQDAPD PIKRCIYQVS CMLCAESMLY HCMSDSEGDF SDPCSCDTSD DKFCLRWLAL VALSFIVPCM CCYVPLRMCH RCGEACGCCG GKHKAAG
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SPRED1
Purity
>80%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
52.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-51864.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>80%, by SDS-PAGE

Alternate Names for Recombinant Human SPRED1 Protein

  • EVH1/Sprouty domain containing protein
  • FLJ33903
  • hSpred1
  • NFLS
  • SPRED1
  • spred-1
  • sprouty-related, EVH1 domain containing 1
  • sprouty-related, EVH1 domain-containing protein 1
  • suppressor of Ras/MAPK activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SPRED2 was detected in immersion fixed PC‑12 rat adrenal pheochromocytoma cell line using Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
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EphB2 was detected in immersion fixed paraffin-embedded sections of human esophageal squamous cell carcinoma using Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Immunocytochemistry/Immunofluorescence: VASP Antibody [NBP1-85792] - Staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: VASP Antibody [NBP1-85792] - Staining in human small intestine and skeletal muscle tissues using anti-VASP antibody. Corresponding VASP RNA-seq data are presented for the same tissues.
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SCF/c-kit Ligand [Unconjugated]
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Publications for SPRED1 Recombinant Protein (NBP2-51864)(1)

We have publications tested in 1 confirmed species: Human.


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Bioinformatics

Gene Symbol SPRED1