SPRED2 Antibody

Images

 
Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) followed by ...read more
SPRED2 was detected in immersion fixed PC‑12 rat adrenal pheochromocytoma cell line using Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

SPRED2 Antibody Summary

Immunogen
E. coli-derived recombinant human SPRED2
Lys117-Ser297
Accession # Q7Z698
Specificity
Detects human SPRED2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SPRED1 is observed and less than 3% cross-reactivity with rhSPRED4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SPRED2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPRED2 Antibody

  • FLJ31917
  • MGC163164
  • SPRED2
  • Spred-2related sequence
  • sprouty-related, EVH1 domain containing 2
  • sprouty-related, EVH1 domain-containing protein 2

Background

SPRED2 (Sprouty-related protein with an EVH1 domain-2) is a 47-53 kDa member of the SPRED family of regulatory molecules. In human, it is found in keratinocytes, intestinal columnar epithelium, and salivary duct epithelium. It inhibits receptor tyrosine kinase (RTK) signaling by blocking Ras activation of Raf. This is achieved by inducing the lysosomal degradation of RTKs following SPRED2 binding to NBR1. Human SPRED2 is 418 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 5-122), a central KBD domain that binds to the SCFR (aa 201-257), and a C-terminal Sprouty‑related domain that mediates homodimerization, and heterodimerization with SPRED1 (aa 308-416). SPRED is ubiquitinated, and undergoes phosphorylation on Ser, Thr and Tyr residues. There is one splice variant that shows a six aa substitution for aa 1-9. Over aa 117-297, human SPRED2 shares 84% aa identity with mouse SPRED2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5067
Species: Hu
Applications: WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP2-00555
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-87914
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
236-EG
Species: Hu
Applications: BA
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
MAB4540
Species: Hu, Mu, Rt
Applications: IHC, WB
NBP1-82956
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
H00010253-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-13304
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-15071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP2-66840
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-41091
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF4538
Species: Hu, Mu, Rt
Applications: WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AF743
Species: Mu
Applications: CyTOF-ready, Flow, WB
AF4819
Species: Hu
Applications: WB, ICC/IF

Publications for SPRED2 Antibody (AF4819) (0)

There are no publications for SPRED2 Antibody (AF4819).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SPRED2 Antibody (AF4819) (0)

There are no reviews for SPRED2 Antibody (AF4819). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SPRED2 Antibody (AF4819) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SPRED2 Products

Bioinformatics Tool for SPRED2 Antibody (AF4819)

Discover related pathways, diseases and genes to SPRED2 Antibody (AF4819). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SPRED2 Antibody (AF4819)

Discover more about diseases related to SPRED2 Antibody (AF4819).
 

Pathways for SPRED2 Antibody (AF4819)

View related products by pathway.

PTMs for SPRED2 Antibody (AF4819)

Learn more about PTMs related to SPRED2 Antibody (AF4819).

Blogs on SPRED2

There are no specific blogs for SPRED2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SPRED2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SPRED2
Uniprot