SPINK1 Overexpression Lysate (Native)

Images

 
Western Blot: SPINK1 Overexpression Lysate (Adult Normal) [NBL1-16405] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPINK1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Host
HEK293

Order Details

SPINK1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206574 and based on accession number NM_003122, NP_003113. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens serine peptidase inhibitor, Kazal type 1 (SPINK1), mRNA.
Epitope tag: C-terminal Myc/DDK
Expression cell line: Human HEK293T cell line
Host
HEK293
Gene
SPINK1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.
You will receive the lysate (100ug), and an empty vector negative control (100 ug).
1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 ug empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Theoretical MW
8.51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SPINK1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix,1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20C before shipping.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SPINK1 Overexpression Lysate (Native)

  • pancreatic secretory trypsin inhibitor
  • PCTT
  • PCTTSpink3
  • PSTI
  • PSTISerine protease inhibitor Kazal-type 1
  • serine peptidase inhibitor, Kazal type 1
  • serine protease inhibitor, Kazal type 1
  • SPINK1
  • Spink3
  • TATI
  • TATITumor-associated trypsin inhibitor

Background

The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SPINK1 Lysate (NBL1-16405) (0)

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Video Protocols

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Bioinformatics

Gene Symbol SPINK1
Entrez