SPFH2 Antibody

Images

 
Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot ...read more
Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SPFH2 Antibody Summary

Immunogen
Peptide with sequence C-EDEPLETATKEN corresponding to C-Terminus according to NP_009106.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_009106) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ERLIN2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:2000
Application Notes
WB: Approx. 43 kDa band observed in human breast lysates and in lysates of human breast cancer (PMID: 17502376).
IHC: Paraffin embedded Human Breast.
Positive Control
Breast Lysate (NB820-59203)
Publications
Read Publications using NB100-1884.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat. Chinese Hamster reactivity reported in scientific literature (PMID: 21343306).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPFH2 Antibody

  • C8orf2SPFH domain-containing protein 2
  • Endoplasmic reticulum lipid raft-associated protein 2
  • ER lipid raft associated 2
  • Erlin-2
  • MGC87072
  • NET32
  • SPFH domain family, member 2
  • SPFH2chromosome 8 open reading frame 2
  • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPFH2 Antibody (NB100-1884)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ERLIN2
Entrez
Uniprot