SPFH2 Antibody

Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, ChHa
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SPFH2 Antibody Summary

• Immunogen: Peptide with sequence C-EDEPLETATKEN corresponding to C-Terminus according to NP_009106.1.
• Specificity: This antibody is expected to recognise isoform 1 (NP_009106) only.
• Predicted Species: Rat (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ERLIN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 43 kDa band observed in human breast lysates and in lysates of human breast cancer (PMID: 17502376).
  IHC: Paraffin embedded Human Breast.
• Control:

  Human Breast Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 21343306).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SPFH2 Antibody

• C8orf2SPFH domain-containing protein 2
• Endoplasmic reticulum lipid raft-associated protein 2
• ER lipid raft associated 2
• Erlin-2
• MGC87072
• NET32
• SPFH domain family, member 2
• SPFH2chromosome 8 open reading frame 2
• Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Background

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPFH2 Antibody (NB100-1884)(4)

Publications using NB100-1884

• Ahmed M. O. Elbatsh, Ali Amin-Mansour, Anne Haberkorn, Claudia Textor, Nicolas Ebel, Emilie Renard, Lisa M. Koch, Femke C. Groenveld, Michelle Piquet, Ulrike Naumann, David A. Ruddy, Vincent Romanet, Julia M. Martínez Gómez, Matthew D. Shirley, Peter Wipfli, Christian Schnell, Markus Wartmann, Martin Rausch, Martine J. Jager, Mitchell P. Levesque, Sauveur-Michel Maira, Eusebio Manchado INPP5A phosphatase is a synthetic lethal target in GNAQ and GNA11-mutant melanomas Nature Cancer 2024-01-17 [PMID: 38233483]
• Jo Y, Sguigna PV, Debose-Boyd RA. Membrane-associated Ubiquitin ligase complex containing gp78 mediates sterol-accelerated degradation of 3-hydroxy-3-methylglutaryl coenzyme a reductase. J Biol Chem. 2011-02-22 [PMID: 21343306]
• Pearce MM, Wang Y, Kelley GG et al. SPFH2 mediates the endoplasmic reticulum-associated degradation of inositol 1,4,5-trisphosphate receptors and other substrates in mammalian cells. J Biol Chem 2007-07-01 [PMID: 17502376]
• Garcia MJ, Pole JC, Chin SF et al. A 1 Mb minimal amplicon at 8p11-12 in breast cancer identifies new candidate oncogenes. Oncogene 2005-08-04 [PMID: 15897872]

Bioinformatics

• Gene Symbol: ERLIN2
• Entrez:
  • 11160(Human)
  • 290823(Rat)
• Uniprot:
  • NP_009106.1.(Human)