Sperm-associated antigen 7 Overexpression Lysate

Images

 
Western Blot: Sperm-associated antigen 7 Overexpression Lysate (Adult Normal) [NBL1-16367] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sperm-associated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Sperm-associated antigen 7 Overexpression Lysate Summary

Description
Transient overexpression lysate of sperm associated antigen 7 (SPAG7) The lysate was created in HEK293T cells, using Plasmid ID RC204174 and based on accession number NM_004890. The protein contains a C-MYC/DDK Tag.
Gene
SPAG7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
25.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Sperm-associated antigen 7 Overexpression Lysate

  • ACRP
  • FSA-1
  • MGC20134
  • sperm associated antigen 7
  • sperm-associated antigen 7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-59904
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87034
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-89425
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-22106
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP1-88523
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB1144
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-77089
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-36528
Species: Hu, Mu
Applications: IP (-), WB
H00050854-P01
Species: Hu
NBP1-79022
Species: Hu, Mu
Applications: WB, IP
H00006757-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB500-248
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47791
Species: Hu
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
NBP2-37364
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC, KD
H00007775-M06
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
AF2228
Species: Hu
Applications: WB, IHC, ICC
NBP2-24530
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P, Flow-IC

Publications for Sperm-associated antigen 7 Lysate (NBL1-16367) (0)

There are no publications for Sperm-associated antigen 7 Lysate (NBL1-16367).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Sperm-associated antigen 7 Lysate (NBL1-16367) (0)

There are no reviews for Sperm-associated antigen 7 Lysate (NBL1-16367). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sperm-associated antigen 7 Lysate (NBL1-16367) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-16367

Additional Sperm-associated antigen 7 Products

Bioinformatics Tool for Sperm-associated antigen 7 Lysate (NBL1-16367)

Discover related pathways, diseases and genes to Sperm-associated antigen 7 Lysate (NBL1-16367). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sperm-associated antigen 7 Lysate (NBL1-16367)

Discover more about diseases related to Sperm-associated antigen 7 Lysate (NBL1-16367).
 

Pathways for Sperm-associated antigen 7 Lysate (NBL1-16367)

View related products by pathway.

Blogs on Sperm-associated antigen 7

There are no specific blogs for Sperm-associated antigen 7, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Sperm-associated antigen 7 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SPAG7
COVID-19 update