SPEN Antibody

Product Discontinued

SPEN Antibody Summary

• Immunogen: Peptide with sequence C-DSEGKMDDKKEDHKE corresponding to internal region according to NP_055816.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPEN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of approx. 402 kDa band according to NP_055816.2.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for SPEN Antibody

• HIAA0929
• KIAA0929nuclear receptor transcription cofactor
• MINTMsx2 interacting nuclear target (MINT) homolog
• RBM15C
• SHARPmsx2-interacting protein
• SMART/HDAC1 associated repressor protein
• SMART/HDAC1-associated repressor protein
• SPEN homolog
• spen homolog, transcriptional regulator (Drosophila)

Background

SPEN encodes a hormone inducible transcriptional repressor. Repression of transcription by this gene product can occur through interactions with other repressors, by the recruitment of proteins involved in histone deacetylation, or through sequestration of transcriptional activators. The product of this gene contains a carboxy-terminal domain that permits binding to other corepressor proteins. This domain also permits interaction with members of the NuRD complex, a nucleosome remodeling protein complex that contains deacetylase activity. In addition, this repressor contains several RNA recognition motifs that confer binding to a steroid receptor RNA coactivator; this binding can modulate the activity of both liganded and nonliganded steroid receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPEN Antibody (NB100-2804)(1)

Publication using NB100-2804

• Hiriart E, Gruffat H, Buisson M et al. Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J Biol Chem 2005-11-04 [PMID: 16129689]

Bioinformatics

• Gene Symbol: SPEN
• Entrez:
  • 23013(Human)
• Uniprot:
  • NP_055816.2(Human)