Mouse SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric)

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ELISA: Mouse SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) [NBP2-76696] - Samples were spiked with high concentrations of Mouse SPARC-like 1/SPARCL1 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Mouse SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) [NBP2-76696] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL

Order Details

Mouse SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156 - 10ng/mL
Sensitivity
0.094ng/mL
Assay Type
Competitive-ELISA
Inter-Assay
CV% < 5.83
Intra-Assay
CV% < 5.47
Spike Recovery
85-108%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SPARCL1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric)

  • Hevin
  • High endothelial venule protein
  • MAST 9
  • MAST9
  • PIG33
  • proliferation-inducing protein 33
  • SC1
  • SPARC like 1
  • SPARCL1
  • SPARC-like 1 (hevin)
  • SPARC-like 1 (mast9, hevin)
  • SPARC-like 1
  • SPARC-like protein 1

Background

Human SPARC-like 1 (SPARCL1), also known as MAST9 or Hevin, is a member of the SPARC protein family. It is a secreted acidic calcium-binding protein of 664 amino acids that shows 62% identity with SPARC. SPARCL1 is a secreted protein, found in the extracellular matrix. It is expressed at high levels in high endothelial venules (HEV) in lymphoid tissues. SPARCL1 inactivation is a common event in tumors of epithelial origin and levels are down-regulated in a variety of tumors. It may have tumor suppressor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SPARCL1