SPAG6 Antibody (3E3) - Azide and BSA Free

Western Blot: SPAG6 Antibody (3E3) [H00009576-M01] - SPAG6 monoclonal antibody (M01), clone 3E3 Analysis of SPAG6 expression in A-431.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 3E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SPAG6 Antibody (3E3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SPAG6 Antibody (3E3) - Azide and BSA Free (H00009576-M01) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: SPAG6 (NP_036575, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRTLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKCDILPQLVYSLAEQNRFYK
• Specificity: SPAG6 (3E3)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPAG6
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPAG6 Antibody (3E3) - Azide and BSA Free

• axoneme central apparatus protein
• DKFZp434I153
• pf16
• Protein PF16 homolog
• Repro-SA-1MGC26276
• sperm associated antigen 6
• Sperm flagellar protein
• sperm-associated antigen 6

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPAG6
• Entrez:
  • 9576(Human)
• OMIM:
  • 605730(Human)
• Uniprot:
  • NP_036575(Human)