SP1 Antibody (JF0950)

Western Blot: SP1 Antibody (JF0950) [NBP2-67159] - Western blot analysis of SP1 on U937 cells using anti-SP1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: SP1 Antibody (JF0950) [NBP2-67159] - Staining SP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SP1 Antibody (JF0950) [NBP2-67159] - Analysis of paraffin-embedded human kidney tissue using anti-SP1 antibody. Counter stained with hematoxylin.

Western Blot: SP1 Antibody (JF0950) [NBP2-67159] - Analysis of SP1 on different lysates using anti-SP1 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela

Immunocytochemistry/Immunofluorescence: SP1 Antibody (JF0950) [NBP2-67159] - Staining SP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SP1 Antibody (JF0950) [NBP2-67159] - Staining SP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: SP1 Antibody (JF0950) [NBP2-67159] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-SP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SP1 Antibody (JF0950) [NBP2-67159] - Analysis of paraffin-embedded human colon cancer tissue using anti-SP1 antibody. Counter stained with hematoxylin.

Product Discontinued

SP1 Antibody (JF0950) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SP1 aa 544-588 / 785. (SwissProt: P08047 Human)
• Localization: Nucleus, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SP1 Antibody (JF0950)

• Sp1 transcription factor
• specificity protein 1
• transcription factor Sp1
• TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP1