SP-D Overexpression Lysate (Native)

Images

 
Western Blot: Surfactant protein D Overexpression Lysate (Adult Normal) [NBL1-15897] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Surfactant protein D.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SP-D Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204746 and based on accession number NM_003019. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens surfactant protein D (SFTPD), mRNA.
Gene
SFTPD

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SP-D

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SP-D Overexpression Lysate (Native)

  • COLEC7collectin-7
  • Collectin 7
  • Collectin-7
  • Lung surfactant protein D
  • PSPD
  • PSP-D
  • SFTP4
  • SFTP4pulmonary surfactant-associated protein D
  • SFTPD
  • SPD
  • SP-D
  • SP-Dpulmonary surfactant apoprotein
  • surfactant protein D
  • surfactant, pulmonary-associated protein D
  • surfactant-associated protein, pulmonary 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-22711
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
MAB4077
Species: Hu
Applications: WB, IHC
NBP1-57977
Species: Hu
Applications: WB, IHC
NBP1-87201
Species: Hu
Applications: WB, IHC, IHC-P
MAB4218
Species: Hu
Applications: WB, IHC
NBP1-22568
Species: Hu
Applications: IHC-P
H00001406-M02
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF
NBP1-84412
Species: Hu
Applications: IHC, IHC-P
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
MAB59151
Species: Mu
Applications: WB
MAB9167
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ICC
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for SP-D Lysate (NBL1-15897) (0)

There are no publications for SP-D Lysate (NBL1-15897).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SP-D Lysate (NBL1-15897) (0)

There are no reviews for SP-D Lysate (NBL1-15897). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SP-D Lysate (NBL1-15897) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SP-D Products

Bioinformatics Tool for SP-D Lysate (NBL1-15897)

Discover related pathways, diseases and genes to SP-D Lysate (NBL1-15897). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SP-D Lysate (NBL1-15897)

Discover more about diseases related to SP-D Lysate (NBL1-15897).
 

Pathways for SP-D Lysate (NBL1-15897)

View related products by pathway.

Research Areas for SP-D Lysate (NBL1-15897)

Find related products by research area.

Blogs on SP-D

There are no specific blogs for SP-D, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SP-D Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol SFTPD
Entrez