SP-D Antibody (12G5) - BSA Free

Product Discontinued

SP-D Antibody (12G5) - BSA Free Summary

• Immunogen: Recombinant neck CRD SP-D adsorbed onto aluminum hydroxide
• Epitope: Not determined
• Specificity: Clone 12G5 is specific for human SP-D
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SFTPD
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: In immunohistochemical staining on paraffin embedded tissues, Clone 12G5 reacts specifically with SP-D. Immunoreactivity can be found in epithelial cells of the lung, skin, small intestine, esophagus, gall-bladder, kidney and urinary bladder.In Western blotting after SDS-PAGE Clone 12G5 reacts strongly with SP-D, both in reduced and non-reduced forms. In Western blotting a dilution guideline of 1/20.000 has proved successful.Clone 12G5 can be used for ELISA with Ca++ present in the buffer.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for SP-D Antibody (12G5) - BSA Free

• COLEC7collectin-7
• Collectin 7
• Collectin-7
• Lung surfactant protein D
• PSPD
• PSP-D
• SFTP4
• SFTP4pulmonary surfactant-associated protein D
• SFTPD
• SPD
• SP-D
• SP-Dpulmonary surfactant apoprotein
• surfactant protein D
• surfactant, pulmonary-associated protein D
• surfactant-associated protein, pulmonary 4

Background

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFTPD