SP-B/Surfactant Protein B Antibody

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, IHC, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

SP-B/Surfactant Protein B Antibody Summary

• Immunogen: The polyclonal antibody is raised against human-based 60-amino acid synthetic SP-B polypeptide.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFTPB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for SP-B/Surfactant Protein B Antibody

• 18 kDa pulmonary-surfactant protein
• 6 kDa protein
• PSP-B
• pulmonary surfactant-associated protein B
• Pulmonary surfactant-associated proteolipid SPL(Phe)
• SFTB3
• SFTP3
• SFTPB
• SMDP1
• SP-B surfactant, pulmonary-associated protein B
• SP-B
• surfactant protein B

Background

The polyclonal antibody recognizes the human surfactant protein B (SP-B). There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant. SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behaviour in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS). The polyclonal antibody is raised against human-based 60-amino acid synthetic SP-B polypeptide. The antibody recognizes proteins of 42, 25 and 18 kD under nonreducing conditions. The 18 kD form is reduced to 7.7-8 kD in the presence of beta-mercaptoethanol. The polyclonal antibody is cross reactive with mouse, bovine and pig SP-B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SP-B/Surfactant Protein B Antibody (NBP3-11280)(1)

Publication using NBP3-11280

• Oh C, Jeon O, Han J et al. Pulmonary Surfactant-Based Paclitaxel-Loaded Nanovesicles for Inhalation Therapy of Lung Adenocarcinoma ACS Applied Nano Materials 2023-02-24

Bioinformatics

• Gene Symbol: SFTPB