SOX9 Antibody (2A2)

Western Blot: SOX9 Antibody (2A2) [H00006662-M01] - Analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M01), clone 2A2.Lane 1: SOX9 transfected lysate(63.639 KDa).Lane 2: Non-transfected lysate.

ELISA: SOX9 Antibody (2A2) [H00006662-M01] - Detection limit for recombinant GST tagged SOX9 is 0.03 ng/ml as a capture antibody.

Product Discontinued

SOX9 Antibody (2A2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SOX9 (NP_000337, 400 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP
• Marker: Sertoli Cell Marker
• Specificity: SOX9 - SRY (sex determining region Y)-box 9 (campomelic dysplasia, autosomal sex-reversal)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX9
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25224227). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SOX9 Antibody (2A2)

• campomelic dysplasia, autosomal sex-reversal
• CMD 1
• CMD1
• CMPD1
• SOX9
• SRA1SRY (sex-determining region Y)-box 9 protein
• SRY (sex determining region Y)-box 9
• SRY-related HMG-box, gene 9
• transcription factor SOX-9

Background

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX9 Antibody (H00006662-M01)(4)

Publications using H00006662-M01

• Zhao L, Arsenault M, Ng ET et al. SOX4 regulates gonad morphogenesis and promotes male germ cell differentiation in mice. Dev Biol 2017-03-01 [PMID: 28118982]
• Rios-Rojas C, Spiller C, Bowles J, Koopman P. Germ cells influence cord formation and Leydig cell gene expression during mouse testis development. Dev Dyn 2015-12-05 [PMID: 26637965]
• Wainwright EN, Svingen T, Ng ET et al. Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice. Dev Biol. 2014-11-15 [PMID: 25224227] (Mouse)
• Csaki C, Matis U, Mobasheri A et al. Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol 2007-12-01 [PMID: 17922135]

Bioinformatics

• Gene Symbol: SOX9
• Entrez:
  • 6662(Human)
• OMIM:
  • 114290(Human)
• Uniprot:
  • NP_000337(Human)