SOX6 Antibody

Western Blot: SOX6 Antibody [NBP2-20458] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunoprecipitation: SOX6 Antibody [NBP2-20458] - IP samples: HeLa whole cell extract A. Control with 5 ug of preimmune Rabbit IgG B. Immunoprecipitation of SOX6 protein by 5 ug SOX6 antibody [N1C3] 7.5 % SDS-PAGE. The immunoprecipitated SOX6 protein was detected by SOX6 antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

• Reactivity: Hu, Mu, Rt, Bv, Ch
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SOX6 Antibody Summary

• Description: Novus Biologicals Rabbit SOX6 Antibody (NBP2-20458) is a polyclonal antibody validated for use in WB and IP. Anti-SOX6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human SOX6 Recombinant protein.
• Predicted Species: Mouse (96%), Rat (91%), Chicken (90%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOX6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SOX6 Antibody

• HSSOX6
• SOX6
• SOXD
• SRY (sex determining region Y)-box 6
• SRY-box containing gene 6
• transcription factor SOX-6

Background

This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SOX6 Antibody (NBP2-20458)(1)

Publication using NBP2-20458

• Liu YF, Zhang M, Shan YJ et al. Transcriptome sequencing analysis of the role of miR-499-5p and SOX6 in chicken skeletal myofiber specification Frontiers in Genetics 2022-09-15 [PMID: 36186474] (Western Blot)

Bioinformatics

• Gene Symbol: SOX6
• Uniprot:
  • P35712(Human)