SorLA Antibody

Product Discontinued

SorLA Antibody Summary

• Immunogen: Synthetic peptide, C-KDLIRKTDRSYKVK, representing the internal region of the sequence according to NP_003096.1
• Epitope: KDLIRKTDRSYKVK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SORL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:32000
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 75kDa band in Human Brain (Cerebellum, Frontal Cortex, Hippocampus) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 248kDa according to NP_003096.1. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for SorLA Antibody

• C11orf32
• chromosome 11 open reading frame 32
• FLJ21930
• FLJ39258
• gp250
• Low-density lipoprotein receptor relative with 11 ligand-binding repeats
• LR11
• LR11LDLR relative with 11 ligand-binding repeats
• LRP9
• mosaic protein LR11
• SORL1
• SorLA
• SorLA-1SORLA
• sortilin-related receptor
• sortilin-related receptor, L(DLR class) A repeats containing
• sortilin-related receptor, L(DLR class) A repeats-containing
• Sorting protein-related receptor containing LDLR class A repeats

Background

This gene encodes a protein that belongs to the families of vacuolar protein sorting 10 (VPS10) domain-containing receptor proteins, of low density lipoprotein receptor (LDLR) proteins, and of fibronectin type III repeats proteins. In addition to VPS10, LDLR and fibronectin type 3 domains, this protein also includes an epidermal growth factor precursor-like module, a single transmembrane segment and a cytoplasmic tail with features similar to endocytosis and sorting-competent receptors. Members of the VPS10 domain-containing receptor family are large with many exons but the CDS lengths are usually less than 3700 nt; this gene is an exception to the pattern with a CDS length greater than 6600 nt. Very large introns typically separate the exons encoding the VPS10 domain; the remaining exons are separated by much smaller-sized introns. The encoded protein is mainly intracellular and localizes in the paranuclear compartment. It is synthesized as a preproprotein, and when the propeptide is still attached, no binding occurs to the VPS10 domain. This gene is strongly expressed in the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SORL1
• Entrez:
  • 6653(Human)
• Uniprot:
  • NP_003096.1(Human)