SorLA Antibody [Unconjugated]

Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorLA at approximately 330 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SorLA was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human/Mouse SorLA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Product Discontinued

SorLA Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse SorLA
  Ser82-Asn367
  Accession # Q92673
• Specificity: Detects human and mouse SorLA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SORL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorLA Antibody [Unconjugated]

• C11orf32
• chromosome 11 open reading frame 32
• FLJ21930
• FLJ39258
• gp250
• Low-density lipoprotein receptor relative with 11 ligand-binding repeats
• LR11
• LR11LDLR relative with 11 ligand-binding repeats
• LRP9
• mosaic protein LR11
• SORL1
• SorLA
• SorLA-1SORLA
• sortilin-related receptor
• sortilin-related receptor, L(DLR class) A repeats containing
• sortilin-related receptor, L(DLR class) A repeats-containing
• Sorting protein-related receptor containing LDLR class A repeats

Background

SorLA (Sorting protein-related receptor containing LDLR class A repeats; also LR11 and SorL1) is a 250-330 kDa, monomeric member of the SORL1 family of proteins. It is expressed on the cell surface (10%) and in Golgi/ER membranes (90%) of neurons and vascular smooth muscle cells. SorLA binds apoE-rich lipoproteins plus the 39 kDa RAP. It also acts as an intracellular sorting receptor for APP. In this context, it blocks transport of APP into compartments that exhibit beta -secretase activity, thus interfering with A beta peptide generation. Human SorLA is a type I transmembrane glycoprotein 2214 amino acids (aa) in length. It contains a 28 aa signal sequence, a 53 aa propeptide, and a 2133 aa mature molecule. The mature molecule contains a 2056 aa extracellular domain (ECD) (aa 82-2137) and a short 56 aa cytoplasmic tail. The ECD is highly modular, containing five BNR/Asp-box repeats (aa 136-573), five LDLR class B repeats (aa 800-1013), one EGF-like domain (aa 1026‑1072), 11 LDLR class A domains (aa 1076-1551) and six FN type III repeats (aa 1554-2023). Multiple potential splice variants exist. There are alternative start sites at Met1057 and Met1155, plus a 63 aa substitution for aa 1-1153 and a 19 aa substitution for aa 1-1404. Over aa 82-367, human SorLA shares 93% aa identity with mouse SorLA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SorLA Antibody (AF5699)(1)

Publication using AF5699

• K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi Prions amplify through degradation of the VPS10P sorting receptor sortilin PLoS Pathog., 2017;13(6):e1006470. 2017 [PMID: 28665987] (Western Blot, Mouse)

Review for SorLA Antibody (AF5699) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: cynthia morimoto IHC-Fr Mouse 12/19/2014

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain cryosections
• Species: Mouse

Bioinformatics

• Gene Symbol: SORL1
• Uniprot:
  • Q92673()