Sonic Hedgehog/Shh Antibody (SC05-28)

Western Blot: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Sonic Hedgehog/Shh antibody. Counter stained with hematoxylin.

Flow Cytometry: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antib

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (SC05-28) [NBP2-67603] - Analysis of paraffin-embedded rat liver tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.

Product Discontinued

Sonic Hedgehog/Shh Antibody (SC05-28) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Sonic Hedgehog/Shh aa 258-307 / 462. (SwissProt: Q15465 Human)
• Localization: Secreted, Cell membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SHH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Sonic Hedgehog/Shh Antibody (SC05-28)

• HHG1
• HHG-1
• HLP3
• HPE3
• MCOPCB5
• MCOPCB5sonic hedgehog (Drosophila) homolog
• Shh
• ShhNC
• SMMCI
• SMMCIsonic hedgehog homolog (Drosophila)
• sonic hedgehog homolog
• sonic hedgehog protein
• Sonic Hedgehog
• TPT
• TPTPS

Background

Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal (1). Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene (2). Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo (3). A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHH