Recombinant Human Soluble Liver/Pancreas Antigen His Protein

SDS-Page: Recombinant Human Soluble Liver/Pancreas Antigen His Protein [NBP1-50890] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human Soluble Liver/Pancreas Antigen His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-501 of Human Soluble Liver/Pancreas Antigen
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SEPSECS
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 57.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.5), 40% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Soluble Liver/Pancreas Antigen His Protein

• EC 2.9.1.2
• EC 2.9.1.n1
• Liver-pancreas antigen
• LPDKFZp434B1417
• MGC161491
• O-phosphoseryl-tRNA(Sec) selenium transferase
• Sec synthase
• Selenocysteine synthase
• Selenocysteinyl-tRNA(Sec) synthase
• Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
• SepSecS
• Sep-tRNA:Sec-tRNA synthase
• SLA/LP autoantigen
• SLA/LP
• SLA-p35
• SLAUGA suppressor tRNA-associated protein
• Soluble liver antigen
• soluble liver antigen/liver pancreas antigen
• tRNA(Ser/Sec)-associated antigenic protein
• TRNP48

Background

SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis. Recombinant human SEPSECS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEPSECS