Soluble Liver/Pancreas Antigen Overexpression Lysate

Product Discontinued

Soluble Liver/Pancreas Antigen Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC224350 and based on accession number NM_153825. The protein contains a C-terminal DDK tag.
• Specificity: Homo sapiens Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), transcript variant 2, mRNA.
• Gene: SEPSECS

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 48.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Soluble Liver/Pancreas Antigen Overexpression Lysate

• EC 2.9.1.2
• EC 2.9.1.n1
• Liver-pancreas antigen
• LPDKFZp434B1417
• MGC161491
• O-phosphoseryl-tRNA(Sec) selenium transferase
• Sec synthase
• Selenocysteine synthase
• Selenocysteinyl-tRNA(Sec) synthase
• Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
• SepSecS
• Sep-tRNA:Sec-tRNA synthase
• SLA/LP autoantigen
• SLA/LP
• SLA-p35
• SLAUGA suppressor tRNA-associated protein
• Soluble liver antigen
• soluble liver antigen/liver pancreas antigen
• tRNA(Ser/Sec)-associated antigenic protein
• TRNP48

Background

The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA(sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA(sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA(sec). SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor (Palioura et al., 2009 (PubMed 19608919)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SEPSECS