SOCS-3 Antibody (OTI3D3) [PE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI3D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

SOCS-3 Antibody (OTI3D3) [PE] Summary

• Immunogen: Full length recombinant protein of human SOCS-3 (NP_003946) produced in HEK293T cells.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOCS3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SOCS-3 Antibody (OTI3D3) [PE]

• ATOD4
• CIS3
• CIS3CIS-3
• Cish3
• cytokine-induced SH2 protein 3
• Cytokine-inducible SH2 protein 3
• SOCS3
• SOCS-3
• SOCS-3STAT-induced STAT inhibitor 3
• SSI-3
• SSI-3ATOD4
• SSI3MGC71791
• suppressor of cytokine signaling 3

Background

Accumulating evidence has demonstrated that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules termed SOCS (Suppressor of Cytokine Signaling) (1-3). To date, there are eight members of SOCS family that have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally, the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that has been called the SOCS box (4). The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism (5). Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 3 is an important regulator of fetal liver hematopoiesis. It is also involved in a broad spectrum of cytokines, e.g. IL-2, IL-3, IL-4, IL-6, Epo, Prolactin, and GH (6-8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS3