SOCS-1 Overexpression Lysate

Images

 
Western Blot: SOCS1 Overexpression Lysate (Adult Normal) [NBL1-16325] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOCS1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SOCS-1 Overexpression Lysate Summary

Description
Transient overexpression lysate of suppressor of cytokine signaling 1 (SOCS1) The lysate was created in HEK293T cells, using Plasmid ID RC220847 and based on accession number NM_003745. The protein contains a C-MYC/DDK Tag.
Gene
SOCS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
23.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SOCS-1 Overexpression Lysate

  • CIS1
  • Cish1
  • JAB
  • JABTec-interacting protein 3
  • JAK binding protein
  • JAK-binding protein
  • SOCS1
  • SOCS-1
  • SOCS-1CIS1
  • SSI-1
  • SSI-1CISH1
  • SSI1cytokine-inducible SH2 protein 1
  • STAT induced SH3 protein 1
  • STAT-induced STAT inhibitor 1
  • suppressor of cytokine signaling 1
  • TIP3
  • TIP-3
  • TIP3Cish1

Background

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30943
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-27210
Species: Hu, Fe, Pm
Applications: WB, IHC, IHC-P
NBP2-59451
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
7270-IL/CF
Species: Hu
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NB400-141
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
NBP2-22471
Species: Hu, Mu, Rt, Pm, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
NBP2-75671
Species: Hu
Applications: WB, ICC/IF, IP
6507-IL/CF
Species: Hu
MAB4260
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow, KO
NBP2-00622
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NBP2-27406
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBL1-16325
Species: Hu
Applications: WB

Publications for SOCS-1 Lysate (NBL1-16325) (0)

There are no publications for SOCS-1 Lysate (NBL1-16325).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SOCS-1 Lysate (NBL1-16325) (0)

There are no reviews for SOCS-1 Lysate (NBL1-16325). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOCS-1 Lysate (NBL1-16325) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-16325

Additional SOCS-1 Products

Bioinformatics Tool for SOCS-1 Lysate (NBL1-16325)

Discover related pathways, diseases and genes to SOCS-1 Lysate (NBL1-16325). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SOCS-1 Lysate (NBL1-16325)

Discover more about diseases related to SOCS-1 Lysate (NBL1-16325).
 

Pathways for SOCS-1 Lysate (NBL1-16325)

View related products by pathway.

PTMs for SOCS-1 Lysate (NBL1-16325)

Learn more about PTMs related to SOCS-1 Lysate (NBL1-16325).
 

Research Areas for SOCS-1 Lysate (NBL1-16325)

Find related products by research area.

Blogs on SOCS-1

There are no specific blogs for SOCS-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SOCS-1 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol SOCS1